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NO
News is Good News - Detection, Measurement and Quantification
of Nitric Oxide: Tools for NO Research
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23rd
March 2007
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BioPark,
Hertfordshire
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Chair:
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Nicholas
Warrick
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Department
of Cardiovascular Medicine
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University
of Oxford
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Nitric
oxide (NO), generated by the enzyme nitric oxide synthase
(NOS) plays a key role in a diverse range of physiological
and pathophysiological conditions within the cardiovascular,
immune, reproductive and nervous systems. Since NO is
a small, diffusible, highly reactive free radical with
a short half-life, and is present in low concentrations,
real-time detection of NO is extremely difficult.
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This
first EuroSciCon Nitric Oxide meeting
examined methods used to detect and visualise NO-producing
cells. Furthermore, direct and indirect techniques used
to measure NO were examined, including among others,
the measurement of NOS enzymatic activity, electron
paramagnetic resonance (EPR) and NO-sensitive electrodes.
Finally, tools that facilitate NO research, such as
NOS inhibitors were examined.
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