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 DUO773

  
  Dual Microprobe System 2-channel intracellular amplifier  

Headstage — Two gold-plated, epoxy sealed miniature active probes can be positioned directly to the measurement site. Microelectrode holders containing an Ag/AgCl electrochemical half-cells plug directly into the probes. Stray capacitance can be reduced by placing the included driven guard shield over the microelectrode holder at the end of the probe.

Capacity Compensation — Channel A can compensate up to 10 pF of electrode shunt capacity and Channel B can compensate up to 50 pF.

Tickler Circuit — Assists in cell penetration. The frequency and amplitude of the oscillations may be varied for differences in membrane thickness or cell size. The duration of tickle can be controlled either by using the momentary switch, a foot switch, or by applying a signal to the remote tickler input.

Active Filters — Low pass settings on a -40 dB/decade active filter vary the cut-off from 1 to 30 kHz. Either probe or bridge outputs may be selected for filtering.

Current Injection — Channel B can eject current through the microelectrode by applying a command signal to the stimulus input connector; the resulting output from the probe will then be a constant current replica of the input signal. Two ranges of current delivery are provided: 50 nA and 500 nA or by an external source. This source can be useful for delivering hyperpolarizing currents to stabilize the cell membrane potential and as a holding current for microiontophoresis.

Compliance Alarm — When the electrode voltage exceeds the probe input maximum allowed voltage, an audible over-compliance alarm will sound.

Bridge Balance — Subtracts the excess electrode voltage associated with delivering current through the recording micropipette. Electrode resistances up to 1000 M can be balanced in two ranges. The balanced signal is available from x10 or x50 front panel output connectors.

Independent Outputs — The Duo773 has an output for each probe independent of gain filtering or balancing. In addition the Duo773 has a 10x and a 50x output for easy integration to most data acquisition programs.

Digital Meter — The Duo773 comes complete with a 31/2-digit display for monitoring injection current or the voltages for either probe (single ended or differential).
 
 
 

 

ACTIVE PROBE INPUT IMPEDANCE
SPECIFICATIONS
HEADSTAGE (PROBE)
712P 715P
>1011 Ohms 1015 Ohms
GAIN x1, x10 x1
OUTPUT RESISTANCE 100 Ohms 100 Ohms
OUTPUT VOLTAGE RANGE ±10 V< ± 10V
MAXIMUM INPUT VOLTAGE ±15 V ±15 V
PROBE LEAKAGE CURRENT 5 X 10-12 A 10-14 A
DC POSITION ADJUST RANGE ± 300 mV ± 300 mV
ELECTRODE RESISTANCE TEST CURRENT 1 nA 1 pA, 1 nA selectable
INPUT CAPACITY COMPENSATION +10 to -50 pF 0 to -10 pF
NOISE
Input shorted 50 µV p-p 10kHz bandwidth 50µV p-p 10kHz bandwidth
20 M Ohms carbon resistor 200 µV p-p 10kHz bandwidth 200µV p-p10kHzbandwidth
RISE TIME
10-90% direct input small signal 1 µs, typical
10-90% through 20 M (-C "on") 25 µs, typical
CURRENT INJECTION (712P only)**
Internal DC Current ± 50 nA low range, ± 500 nA high range
Externally commanded Current ± 500nA low range, ± 5 µA high range
External current command factor 20 mV/nA low range, 2 mV/nA high range
Current monitor 100 mV/nA low range, 10 mV/nA high range
Compliance 3V low range, 10V high range
Bridge balance 0-100 M, 0-1000 M Ohms
Bridge amplifier gain x 10, x 50
LOW PASS FILTER 40 dB/decade, continuously variable 1-30 kHz
METER SECTION
Display 3.5-digit LED
Ranges 200 mV, 2000 mV, 20 V, 200 nA, 2000 nA
Accuracy and resolution 1 digit
DIMENSIONS
Instrument 17 x 5.25 x 10 in. (43 x 13 x 25 cm)
Probe Diameter: 12 mm Length: 34 mm
POWER 95-135 V or 220-240 V, 50/60 Hz
SHIPPING WEIGHT 15 lb (7 kg)

 

MODELS
773 Duo 773 Electrometer

 
| Rack Mounts | Reference Electrodes | Cables and Connectors | Manual 
                             Note that WPI Equipment is NOT Designed for Human Use

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