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Real-time detection and measurement of a variety of
redox-reactive species is fast and easy using the
electrochemical (amperometric) detection principle
employed in the new TBR4100. This optically isolated
four-channel free radical analyzer has ultra low noise
and independently operated channels.
For use with WPI’s wide range of nitric oxide, hydrogen
peroxide, hydrogen sulphide and oxygen sensors, the
TBR4100 can measure four different species
simultaneously in the same preparation. Simply plug a
sensor into any one of the input channels on the front
panel and select the current range. Poise voltage can be
selected from a range of values tuned for optimal
response from WPI sensors. An independent output for
real-time monitoring of temperature is also included.
The TBR4100 analyzer utilizes PC-based data
acquisition via our Lab-Trax interface; data traces are displayed and recorded in real-time. The Data- Trax software comes pre-configured for single or multiple electrode recording; filters, gains, and smoothing are all set for optimal results. Data can be viewed making adjustments to smoothing and filter settings without affecting the original stored raw data. Electrode calibration from multiple concentration readings can be input into the software's Multipoint Calibration utility which quickly provides a plot and slope calculation for electrode sensitivity determination. Alternately, the Lab-Trax T series data interface can be used for providing simultaneous acquisition of Free Radical data along with other physiological data (ECG, HR, BP, etc.) as each of the four input channels has its own independent input amplifier, filters, and 24-bit converter.
KEY POINTS
- Real-time detection using electrochemical
microsensors.
- Integrated system includes one temperature sensor,
your choice of two additional sensors, and a start-up
kit.
- Measure up to four different species in the same
preparation or simultaneous measurement in four
different preparations.
- Current measurement range from 300 fA to 10 μA (four
ranges) permits wide dynamic range for detection.
- Wide bandwidth allows recording of fast events.
- Measure nitric oxide from < 0.3 nM to 100 μM.
- Measure hydrogen peroxide < 10 nM to 100 mM.
- Measure hydrogen sulphide
- Measure glucose
- Measure oxygen from 0.1% to 100%.
- Isolated architecture allows Lab-Trax interface to
simultaneously measure free radical and independent
analogue data (i.e., ECG, BP, etc.) data on any channel.
Nitric
Oxide Sensors
TBR4100 is fully compatible with all WPI ISO-NO
series of NO sensors. The 2.0 mm ISO-NOP can be plugged
directly into the front control panel of TBR4100.
The nitric oxide micro sensors require the use of the microsensor cable (#15810), which can then be plugged
directly into the front control panel of the
instrument.
Oxygen
Sensors
Two oxygen sensors are currently available. The
ISO-OXY-2 is a 2.0 mm stainless steel sensor, with
replaceable membrane sleeves (#5378) and an internal
refillable electrolyte (#7326). The sensor is similar
in design to WPI's popular OXEL-1
oxygen sensor.
The ISO-OXY-100 is a 100 micron tip diameter oxygen
sensor. The sensor design is based on a flexible
activated carbon fibre sensing electrode coated with a
proprietary membrane that enhances oxygen detection.
| OXYGEN SENSOR
SPECIFICATIONS |
|
ISO-OXY-2 |
ISO-OXY-100 |
| APPLICATION |
Cell Cultures, etc. |
Tissue/Microvessels |
| SENSOR DIAMETER |
2.0 mm |
100 micron |
| RESPONSE TIME |
<10 SEC (90%) |
<5 SEC (90%) |
| DETECTION LIMIT |
0.1 % to 100% |
0.1 % to 100% |
| DRIFT |
<1%/min |
<1%/min |
| SENSITIVITY |
N/A |
N/A |
| PHYSIOLOGICAL INTERFERENCE |
none |
Contact WPI |
S-Nitrosothiols
Sensing
Several applications describing the use of WPI's nitric
oxide sensors for the detection of S-nitrosothiols
have recently been described (see page 47 for further
information).
Hydrogen Peroxide Sensors
Hydrogen Peroxide is produced in biological systems by
controlled pathways at low concentrations that impact
on cell signalling. At higher concentrations
inflammatory cells produce local intense amounts of
this oxidant to kill pathogens. In the development of
human disease uncontrolled formation of hydrogen
peroxide from the mitochondrial respiratory chain and
enzymes such as xanthine oxidase can occur (Prof.
Victor Darley-Usmar, Univ. of Alabama, pers. comm.).
Despite the recognized importance of this oxidant in
biology real-time measurements at low concentration
have been difficult. The hydrogen peroxide sensors
developed by WPI are designed to complement existing
high sensitivity fluorescent approaches with direct
quantitative measurement in biological samples in the
low nM range.
Two hydrogen peroxide sensors are currently available.
The ISO-HPO-2 is a 2.0 mm stainless steel sensor, with
replaceable membrane sleeves (#600012) and an internal
refillable electrolyte (#100042). The sensor is
designed for use in cell cultures and similar
applications. The graph below Illustrates the
linearity of response of the sensor following
successive increases in hydrogen peroxide
concentration in solution.
The ISO-HPO-100 is a 100 micron tip diameter hydrogen
peroxide micro sensor designed for use in tissues and
similar applications. The sensor design is based on a
flexible activated carbon fibre sensing electrode
coated with a proprietary membrane that enhances
hydrogen peroxide detection.
Both sensors incorporate WPI's proprietary combination
electrode technology whereby the hydrogen peroxide
sensing element and separate reference electrode are
encased within a single Faraday-shielded probe design.
This design has been shown to enhance performance
during measurements and minimizes overall sensor size.
| HYDROGEN PEROXIDE
SENSOR SPECIFICATIONS |
|
ISO-HPO-2 |
ISO-HPO-100 |
| APPLICATION |
Cell Cultures, etc. |
Tissue/Microvessels |
| SENSOR DIAMETER |
2.0 mm |
100 micron |
| RESPONSE TIME |
<5 SEC (90%) |
<5 SEC (90%) |
| DETECTION LIMIT |
<100 nM to 100 mM |
<10 nM to 1 mM |
| DRIFT |
<0.1 pA/min |
<1.0 pA/min |
| SENSITIVITY |
0.2 pA/nM |
1 pA/nM |
| PHYSIOLOGICAL INTERFERENCE |
none |
Contact WPI |
Temperature
Sensor
The temperature sensor (#ISO-TEMP-2) is based on a 2.0
mm tip diameter high quality miniature platinum RTD
(Resistance Temperature Detector) electrode. This
design has been shown to provide greater accuracy,
stability and interchangeability during temperature
measurements than traditional thermistor and
thermocouple sensors.
|
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| SPECIFICATIONS |
|
Number of Amperometric Channels |
4 |
|
Signal Bandwidth |
0-3
Hz |
|
Polarization Voltage (selectable via
rotary switch) |
|
Nitric Oxide |
865
mV |
|
Hydrogen Sulfide |
150
mV |
|
Hydrogen Peroxide |
450
mV |
|
Glucose |
600
mV |
|
Oxygen |
700
mV |
| ADJ
(user adjustable) |
+/-
2500 mV |
| |
|
Polarization Voltage Accuracy |
+/-
5 mV |
|
Polarization Voltage Display Resolution |
+/-
1mV |
|
Current measurement Performance |
|
| Range |
Analog Output |
Noise @ 3Hz * |
Noise @ 0.3 Hz * |
| +/- 10 nA |
1 mV / 1 pA |
< 1 pA |
< 0.3 pA |
| +/- 100 nA |
1
mV / 10pA |
< 7 pA |
< 3 pA |
| +/- 1 μA |
1
mV / 100pA |
< 70 pA |
< 30 pA |
| +/- 10 μA |
1
mV / 1μA |
< 700 pA |
< 300 pA |
*Instrument performance is measured as the (max-min) over 20 seconds
period with open input. Typical values are given at 3 Hz and 0.3 Hz
bandwidth. |
|
Typical sensor performance with TBR4100 |
|
ISO-NOPF100 noise |
0.2
nM NO (<2 pA)** |
|
**Sensor noise is measured as the (max-min)
over a 20 seconds period with the sensor
immersed in 0.1 M CuCl2 solution. |
|
MODELS |
TBR4100 Systems
|
| TBR4100-416 |
Four-Channel Free
Radical Analyzer with Lab-Trax 4/16 Data
Acquisition System Includes TBR4100 analyzer
& power cord, Lab-Trax-4/16 data acquisition
system & USB cable, 4 BNC cables, 3
electrode adapter cables, 1 temperature
probe, 2 sensors of your choice, and sensor
start-up kit(s), if applicable. |
|
| TBR4100-424T |
Four-Channel Free
Radical Analyzer with Lab-Trax 4/24T Data
Acquisition System Includes TBR4100 analyzer
& power cord, Lab-Trax-4/24T data
acquisition system & USB cable, 4 BNC
cables, 3 electrode adapter cables, 1
temperature probe, 2 sensors of your choice,
and sensor start-up kit(s), if applicable |
|
| Nitric
Oxide Accessories |
| 5435 ISONOP |
Startup Kit (recommended with first
purchase) |
 |
| ISO-NOP |
Replacement 2 mm shielded sensor and cable |
|
| ISO-NOPF100 |
100 µm Flexible NO Sensor, pkg of 2 |
|
| ISO-NOPF200 |
200 µm Flexible NO Sensor, pkg of 2 |
|
| ISO-NOP007 |
7 µm Nitric Oxide Sensor (pkg of 3) |
|
| ISO-NOPMC |
Microchip Nitric Oxide Sensor (6-month
warranty) |
|
| ISO-NOP3020 |
30 µm Sensor Tips (2 mm length), pkg of 3
(requires #15810) |
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| ISO-NOP3005 |
30 µm Sensor Tips (0.5 mm fibre), pkg of
3 (requires #15810) |
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| ISO-NOPNM |
100 nm NanoSensor, pkg of 3 (requires
cable #15810) |
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| ISO-NOP30-L |
L-Shaped NO Sensor, pkg of 3 |
|
| 5436 |
Replacement Sleeve Kit for 2 mm sensor,
pkg of 4 |
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| 7325 |
ISO-NO Electrolyte (10 mL) |
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| 7521 |
ISO-NO Electrolyte, CO2-insensitive (10 mL) |
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| 5399 |
T-Adapter Kit (pkg of 3) for ISO-NOP |
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| 7357 |
Nitrite Standard Solution, 1 gram/litre
(100 mL) |
|
| Hydrogen
Peroxide Accessories |
| 600011 |
ISO-HPO Startup Kit (recommended with
first purchase) |
|
| ISO-HPO-2 |
2mm Shielded HPO Sensor & Cable |
|
| ISO-HPO-100 |
100 µm HPO Sensor (requires #15810), pkg
of 3 |
|
| 600012 |
Replacement Sleeve Kit for ISO-HPO-2, pkg
of 4 |
|
| 100042 |
ISO-HPO-2 Electrolyte (10 mL) |
|
| Oxygen
Accessories |
| 5377 |
ISO-OXY Startup Kit (recommended with
first purchase) |
|
| ISO-OXY-2 |
2 mm Shielded Oxygen Sensor & Cable |
|
| ISO-OXY-100 |
100 >
m Oxygen Sensor, pkg of 3 |
|
| 5378 |
Replacement Electrode Sleeve Kit, pkg of 4 |
|
| 7326 |
ISO2 Filling Solution (electrolyte) |
|
| Temperature
Accessories |
| ISO-TEMP-2 |
2 mm Platinum RTD Temperature Sensor
(requires #15810) |
|
| General
accessories |
| 15810 |
Microsensor Adapter Cable |
|
| NOCHM-4 |
Four-Port Closed Chamber |
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| NO
Electrodes 
| Demo
Request
| Manual |
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(0)30 6188845
+45 (0)36 988 355
