This particular range of Glass Bottom Dishes has been discontinued. We now offer an alternative - the FluoroDish.

Conventional plastic dishes and chambers limit the utility of the inverted scope for many applications because the thick plastic bottom requires a long working distance objective available only in lower magnifications. Each WPI dish has a flat (0.17 mm thick) optical quality glass bottom, allowing the use of a much shorter objective working distance, larger numerical aperture (NA) and a higher magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in Image Analysis. The glass bottom dish is particularly suitable for fluorescence imaging which requires lower background scattering of light and reduced intrinsic “auto” fluorescence, that occurs when using standard plastic bottom dishes. The glass bottom permits the use of immersion medium such as water, glycerine or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.
An inner well is created within the dish by the glass bottom and the tissue culture grade plastic that forms the sides of the dish (see figure). All inner wells are approximately 1 mm in depth. Five different glass diameters and two sizes of dishes are available from which to select. There are two well choices for the 35 mm diameter dish: 12 mm or 22 mm glass diameter. The 50 mm dish is offered with a choice of three well sizes: 30 mm, 40 mm, and 39 mm. The 50 mm dish is also available with low side walls to facilitate micromanipulations and injections. All are sterile and available in either a single, medical-style blister-pack or in a sleeve of 20.