WPI-Europe - Microinjection - UltraMicroPump III

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	UltraMicroPump III

	UltraMicroPump III can dispense as little as 600 picoliters per incremental advance of the syringe piston

* See "Reproducible and Efficient Murine CNS Gene Delivery Using Microprocessor Controlled Injector," A.I. Brooks et al., Journal of Neuroscience Methods, 80 (1998) 137-147

With its digital controller, UltraMicroPump III can dispense as little as 600 picoliters per incremental advance of the syringe piston (using a 5 µL syringe). Syringes may be filled externally and then inserted into the pump or filled while mounted in the pump. Fluids injected or withdrawn are held entirely within the micro syringe to maintain a low fluid dead volume. For positioning, the UltraMicroPump III may be attached to any of several WPI micropositioners such as the M3301 (manual), DC3001 (motorized), or any manual stereotaxic manipulator.

Smart Controller

An Integral component in the UMP III system is a microprocessor-based controller, Micro4, which provides an "intelligent" and easy-to-use interface to up to four syringe pumps. Operating parameters are set with the membrane keypad and LCD display. From the keypad the user can select the following functions: set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, rate of delivery, and syringe type as well as synchronize the starting and stopping of any combination of syringe pumps. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start / stop operation.

Computer Control

An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.

UMPIII shown mounted to Stereotaxic frame (not included)

UMPIII ACCEPTS: glass syringes with barrel diameters from 5.5. to 9 mm

SPECIFICATIONS
UMP-III SPECIFICATIONS (based on 5 µl syringe)
TOTAL # OF STEPS	20,000 (63 mm travel)
DISPENSING VOLUME	0.6 nL / step
LINEAR MOTION PER STEP	3.175 microns
WEIGHT	325 g (11.4 oz)
MOUNTING ROD DIAMETER	8.0 mm (5/16 in.)
CONTROLLER POWER REQUIREMENTS	12 V DC; 2 amps (UMC4)
DIMENSIONS	Ø 32 mm x 190 mm (Ø 1.3 in. x 7.5 in.)
MODELS
UMP3-1	UltraMicroPump II (one) and Micro4 Controller
UMP3-2	UltraMicroPump II (two) and Micro4 Controller
UMP3-3	UltraMicroPump II (three) and Micro4 Controller
UMP3-4	UltraMicroPump II (four) and Micro4 Controller
UMP3	UltraMicroPump II (without controller)
MICRO4	Micro4 Controller, Four-Channel

Syringes with Luer Fitting (no needle)
WPI P/N	Volume	Description	UMP2	UMP1	SP310
ILS005LT	5 µL	ILS 5 µL Gas-tight Luer tip	Y	N	N
ILS010LT	10 µL	ILS 10 µL Gas-tight Luer tip	Y	N	N
ILS025LT	25 µL	ILS 25 µL Gas-tight Luer tip	Y	N	N
SGE050TLL	50 µL	SGE 50 µL Gas-tight Teflon Luer Lock	Y	Y	Y
SGE100TLL	100 µL	SGE 100 µL Gas-tight Teflon Luer Lock	Y	Y	Y
SGE250TLL	250 µL	SGE 250 µL Gas-tight Teflon Luer Lock	Y	Y	N
Syringes with Replaceable Bevelled Needles
WPI P/N	Volume	Description	UMP2	UMP1	SP310
SGE0005RN*	0.5 µL	SGE 0.5 µL 23 ga (0.63 mm) 70 mm long	Y	Y	Y
SGE001RN*	1.0 µL	SGE 1.0 µL 26 ga (0.47 mm) 70 mm long	Y	Y	Y
SGE005RN	5 µL	SGE 5 µL 23 ga (0.63 mm) 50 mm long	Y	Y	Y
SGE010RNS	10µL	SGE 10 µL 26 ga (0.47 mm) 50 mm long	Y	Y	Y
SGE025RN	25 µL	SGE 25 µL 25 ga (0.50 mm) 50 mm long	Y	Y	Y
SGE050RN	50 µL	SGE 50 µL 25 ga (0.50 mm) 50 mm long	Y	Y	Y
SGE100RN	100 µL	SGE 100 µL 25 ga (0.50 mm) 50 mm long	Y	Y	Y

Replaceable Needles
RN0005	For syringe SGE0005RN, 23 ga (0.63 mm) 70 mm long
RN001	For syringe SGE001RN, 26 ga (0.47 mm) 70 mm long
RN005	For syringe SGE005RN, 23 ga (0.63 mm) 50 mm long
RN010	For syringe SGE010RN(S), 26 ga (0.47 mm) 50 mm long, 5-pack
RN025	For syringe SGE025RN, 26 ga (0.47 mm) 50 mm long, 5-pack

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