- Environmental chamber.
- Programmable air pressure.
- Memory storage for up to 100 programs.
- Write protection and date stamp for each
program.
- Two symmetrical pipettes with each pull.
- Pre-programmed sample programs for
intracellular and patch pipettes.
- Special programming on request.
- Ramp testself test for establishing
program heat settings when a new filament or
glass is introduced.
- Vacuum fluorescent display.
- Internal memory test.
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- Constant current power supply for
filament and pull solenoid.
- Looping pull cycle for fabrication
of patch type micropipettes.
- Self-contained air supply with
filtration system and humidity control.
- Consistent and reliable electrodes
with tip diameters less than 0.1µm.
- Control over the time and pressure
at which the air is delivered.
- Optimized velocity sensing circuit
for maximized sensitivity and
reproducibility.
- Quality control, SEM photograph of a
tip pulled with each puller; criterion
is tip measurement less than 0.1µm and
typically is ~0.06µm.
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The Model P-97 micropipette
puller is the latest generation of the
Flaming/Brown type puller for
fabrication of micropipettes, patch
pipettes and microinjection needles.
While retaining many of the features on
the Model P-87, the P-97 offers
improvements in mechanical, electronic
and software design. The result is
better control of the pulling process
and a higher degree of reproducibility.
The P-97 combines a proven
mechanical system with a sophisticated,
programmable microprocessor controller.
This programmable control of the pulling
parameters allows the investigator to
design application specific pipettes
from a wide range of glass compositions
and sizes. A number of new features
have been incorporated in the design of
the P-97. One of the most
apparent is the environmental chamber
which houses the area surrounding the
heating filament. The environmental
chamber is designed to minimize the
effect of changing humidity on the
reproducibility of pulled pipettes. A
25% increase in power over the previous
version allows for the use of larger
heating filaments, larger diameter glass
and multi-barrelled glass. The metal jaws
that clamp the heating filament have
also been redesigned to minimize heat
retention. A gas delivery mode switch
provides for extended cooling for large
diameter and multi-barreled glass. A
spring-loaded clamping mechanism has
been added for easier loading of glass.
A vacuum fluorescent display has been
added that allows easy viewing from any
direction. New software improvements
on the P-97 include a display of
the total time that the heat is on to
assist in program development and
troubleshooting. Up to 100 programs can
now be written and stored in memory,
which makes the P-97 suitable for
multiple users. These programs can now
be write-protected, adding security to
prevent programs from being changed or
altered inadvertently. The display shows
the last date and time the program was
written or edited. In addition, the air
pressure is included as a programmable
parameter. The P-97 contains a
ramp test to overcome the difficulties
of program adjustment when a new
filament is installed or to help
characterize a new glass and filament
combination. This test allows for the
rapid adjustment of heat values in
established programs and provides
estimates for establishing heat settings
when using new sizes or compositions of
glass capillaries. As with all Sutter
instruments, the electronics have been
carefully designed to maximize noise
rejection. NOTE: Not currently
for sale to our customers in Germany and Italy
- contact us
for information
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français
+49
(0)30 6188845
+45 (0)36 988 355
P-97
Flaming/Brown Pipette Puller
