World Precision Instruments

Search the site by KEYWORD


Quick Finder


Testimonials

Researcher UCL, Institute of Ophthalmology

I am really impressed by your very high level of customer service. I got a problem of our pump recently and contacted the service. Julian Williams replied me immediately and tried to resolve the issue with good suggestions. Meanwhile, she loaned me a pump which enabled me to carry out my experime...
read more

SYS-PV830

SYS-PV830

Pneumatic PicoPump with vacuum


Choose Quantity
  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

Pneumatic PicoPump with Vacuum

There are 1 images available to view - click to enlarge and scroll through the product gallery.

Instruction Manual
/ Download as PDF

Zebrafish Microinjection Setup
/ Download as PDF

Microinjection Datasheet
/ Download as PDF

  • Regulated Hold, Ejection and Vacuum Pressure
  • Carefully regulated air pressures for securing cells and injecting them with fluid
  • Pressure Input: 0-150 psi
  • Pressure Output: 0.3-90 psi

Intracellular injection

Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI™s PicoPumps use carefully regulated air pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure - positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. A second pressure port maintains a low positive "holding" pressure to the injecting pipette between injection pulses, to prevent fluid up­take through capillary action or diffusion. Timing, ejection pressure, holding pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5 volt monitor output provides a logic-level pulse for your computer or other monitoring device.

PV830 Features

Eject pressure, Hold pressure, and Vacuum are all available, controlled by separate regulators on the front panel. Eject pressure supplies a high-pressure pulse for injecting fluid. Hold pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive. Pressure in the injection pipette is automatically switched between Eject and Hold pressure by a precision timing circuit that controls a solenoid valve. Vacuum is used to fill pipettes from the tip or to secure a floating cell during microinjection. Vacuum is regulated the same way, by a 20-turn knob on the front panel. Vacuum may be switched from regulated vacuum to atmosphere by using the pneumatic toggle on the front panel. Vacuum can also be routed to the eject port.

Ideal for zebrafish microinjection systems.

Specifications

PRESSURE

Pressure Input 0-150PSI
Pressure Output 0.3-90PSI
Lowest Regulated Pressure 12" water
Regulator Accuracy 0.1% (20-turn dial)
Regulator Repeatability 0.05PSI (both hold and eject pressures)
Gauge Accuracy 3% at full scale (both hold and eject pressures)
Input Connector Quick Connect (1/4" OD tubing)
Output Connector Barbed (1/16" ID tubing)
Control Solenid

VACUUM

Vacuum Input 0-30.0" Hg
Vacuum Output 0.2-29.9" Hg
Lowest Regulated Vacuum 3" water
Regulator Accuracy 0.1% (20-turn dial)
Regulator Repeatability 0.03" Hg
Gauge Accuracy 3% at full scale
Input Connector Quick Connect (1/4" OD tubing)
Output Connector Barbed (1/16" ID tubing)
Control Manual
Vent Atmosphere

CONNECTIONS INCLUDED

Input Kit 10' nylon tubing (0.25" OD, 1000PSI), one 1/2" female NPT adapter
Output Kit Two PicoNozzle

PHYSICAL SPECS

Power 95-135V or 220-240V, 50/60Hz
Dimensions 17 x5.25 x 9.5" (43 x 13 x 24cm)
Shipping Weight 14 lb. (6.3kg)


Accessories

M3301R

M3301R

Manual Micromanipulator (right-handed)

View details...

M10

M10

Magnetic Stand

View details...


Choose Quantity
5052

5052

Steel Base Plate. 21.5 x 30 cm. 4.5kg

View details...

PZMIII

PZMIII

Precision Stereo Zoom Binocular Microscope (III) on Post Stand

View details...

Citations

Ewart, M., Kennedy, S., MacMillan, D., & Raja, A. (2014). Altered vascular smooth muscle function in the ApoE knockout mouse during the progression of atherosclerosis. Atherosclerosis. Retrieved from http://www.sciencedirect.com/science/article/pii/S0021915014001129

Hall, J., Jheon, A., Ealba, E., & Eames, B. (2014). Evolution of a developmental mechanism: Species-specific regulation of the cell cycle and the timing of events during craniofacial osteogenesis. Developmental  …. Retrieved from http://www.sciencedirect.com/science/article/pii/S0012160613006118

Huang, B., & Gale, D. (2014). Stent with preferential coating. US Patent 8,709,071. Retrieved from http://www.google.com/patents/US8709071

Kongton, K., McCall, K., & Phongdara, A. (2014). Identification of gamma-interferon-inducible lysosomal thiol reductase (GILT) homologues in the fruit fly< i> Drosophila melanogaster. Developmental & Comparative  …. Retrieved from http://www.sciencedirect.com/science/article/pii/S0145305X14000081

Nagase, M., & Takahashi, Y. (2014). On-Site Energy Supply at Synapses through Monocarboxylate Transporters Maintains Excitatory Synaptic Transmission. The Journal of  …. Retrieved from http://www.jneurosci.org/content/34/7/2605.short

Saralahti, A., & Piippo, H. (2014). Adult zebrafish model for pneumococcal pathogenesis. Developmental &  …. Retrieved from http://www.sciencedirect.com/science/article/pii/S0145305X13002619

Shi, Y., Yao, J., Xu, G., & Taber, L. (2014a). Accepted Manuscript Not Copyedited. Retrieved from http://biomechanical.asmedigitalcollection.asme.org/data/Journals/JBENDY/0/BIO-13-1491.pdf

Shi, Y., Yao, J., Xu, G., & Taber, L. (2014b). Bending of the Looping Heart: Differential Growth Revisited. Journal of  …. Retrieved from http://gasturbinespower.asmedigitalcollection.asme.org/article.aspx?articleid=1829834

Tajima, Y., Takuwa, H., & Nishino, A. (2014). Cerebral hemodynamic response to acute hyperoxia in awake mice. Brain research. Retrieved from http://www.sciencedirect.com/science/article/pii/S0006899314001413

Tang, P., & Watson, G. (2014). Cadherin-23 May Be Dynamic in Hair Bundles of the Model Sea Anemone Nematostella vectensis. PloS one. Retrieved from http://dx.plos.org/10.1371/journal.pone.0086084.g002

Toyoda, H., Saito, M., Sato, H., & Tanaka, T. (2014). Enhanced desensitization followed by unusual resensitization in GABAA receptors in phospholipase C-related catalytically inactive protein-1/2 double-knockout mice. … -European Journal of  …. Retrieved from http://link.springer.com/article/10.1007/s00424-014-1511-5

Wang, X., & Piñol, R. (2014). Optogenetic Stimulation of Locus Ceruleus Neurons Augments Inhibitory Transmission to Parasympathetic Cardiac Vagal Neurons via Activation of Brainstem α1 and. The Journal of  …. Retrieved from http://www.jneurosci.org/content/34/18/6182.short

RelatedItems

SYS-PV820

SYS-PV820

Pneumatic PicoPump

View details...

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

Keep in Touch

We promise NEVER to share your details with anyone. You can opt out at any time.

Downloads

Main CatalogueSurgical Tools
MDS
Order a printed version
Browse Online...
Browse Online...