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ISO-HPO-100-L Hydrogen Peroxide Sensor - 100µm

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ISO-HPO-100 Instruction Manual
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  • For use with Apollo1000, Apollo4000, TBR4100 and TBR1025
  • Requires cable 91580 (sold separate)
  • Response Time: < 5s (90%)
  • Detection Limit: 1nM - 1mM
  • Drift: < 1.0pA/min.
  • Sensitivity: 2 pA/nM
  • Application: L-shaped for tissue bath studies
  • Package of 2


L-Shaped sensor for tissue bath studies

The ISO-HPO-100-L is a unique L-shaped nitric oxide sensor designed specifically for use in tissue bath studies and similar applications (e.g., see WPI's MYOBATH). The shape of the sensor has been engineered to facilitate placement of the electrode within the lumen of the tissue vessel under study.

Sensing Hydrogen Peroxide

Hydrogen Peroxide is produced in biological systems by controlled pathways at low concentrations that impact on cell signaling. At higher concentrations inflammatory cells produce local intense amounts of this oxidant to kill pathogens. In the progress of human disease, uncontrolled formation of hydrogen peroxide from the mitochondrial respiratory chain and enzymes, such as xanthine oxidase, can occur (Prof. Victor Darley-Usmar, Univ. of Alabama, personal communication). Despite the recognized importance of this oxidant in biology real-time measurements at low concentration have been difficult. The hydrogen peroxide sensors developed by WPI are designed to compliment existing high sensitivity fluorescent approaches with direct quantitative measurement in biological samples in the low nM range.


The ISO-HPO-100 is a 100 micron tip diameter hydrogen peroxide micro sensor designed for use in tissues and similar applications. The sensor design is based on a flexible "activated" carbon fiber sensing electrode coated with a proprietary membrane that enhances hydrogen peroxide detection.


These sensors incorporate WPI's proprietary combination electrode technology whereby the hydrogen peroxide sensing element and separate reference electrode are encased within a single Faraday-shielded probe design. This design has been shown to enhance performance during measurements and minimizes overall sensor size.nimizes overall sensor size.





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