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Janice Carter, University of Cambridge

The person in WPI I deal with is Julian Williams and he has been very helpful in sorting out any problems we may have and finding items that we require. He is very pleasant to deal with and I have used WPI for the last 8 years and I am very happy with the service that they provide us.
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EVC4000-2 (ex-demo)

EVC4000-2 (ex-demo)

Epithelial Voltage Clamp and preamps - 2 channel

  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

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  • 2 channels
  • Monitor membrane permeability with voltage clamp technique
  • Clamp up to 4 sample membranes
  • Extremely stable and accurate

Voltage Clamp Technique

EVC4000 employs the voltage clamp technique to monitor membrane permeability as a function of membrane voltage or applied chemicals. When combined with WPI’s patented EKC and EKV cartridge electrodes, EVC4000 can efficiently voltage or current clamp up to four sample membranes simultaneously using safe moderate voltages on the current wire leads.

Safe, low-voltage system

The superior design of the cartridge electrodes makes 100-volt current excursion unnecessary, so this safe, low-voltage system is easier to adjust and use. Extremely stable and accurate, each module, with its companion preamplifier, can operate independently in one of three different modes: Voltage Clamp (VC), Current Clamp (CC), or Open Circuit Potential (PD) measurement.

Control

EVC4000 can be controlled from the front panel of the instrument or from computer generated commands applied at the rear panel of the instrument. A feature unique to EVC4000 is an electronic potentiostat in the preamplifier box that maintains the serosal electrode invariant potential at zero relative to system ground. The preamplifier apparatus actively maintains one surface of the test membrane close to ground potential under all operating conditions.

Specifications

Accessories

Citations

Alcolado, N., Conrad, D., & Poroca, D. (2014). Cystic Fibrosis Transmembrane Conductance Regulator dysfunction in VIP knockout mice. American Journal of …. Retrieved from https://ajpcell.physiology.org/content/ajpcell/early/2014/05/29/ajpcell.00293.2013.full.pdf

Al-Sadi, R., Ye, D., Boivin, M., Guo, S., & Hashimi, M. (2014). Interleukin-6 Modulation of Intestinal Epithelial Tight Junction Permeability Is Mediated by JNK Pathway Activation of Claudin-2 Gene. PloS one. Retrieved from https://dx.plos.org/10.1371/journal.pone.0085345.g012

Bhalay, G., & Budd, E. (2014). Treating diseases mediated by blockade of the epithelial sodium channel with pyrazine-2-carboxamide derivatives. US Patent App. 14/ …. Retrieved from https://www.google.com/patents/US20140171421

Blanchard, E., Zlock, L., Lao, A., & Mika, D. (2014). Anchored PDE4 regulates chloride conductance in wild-type and ΔF508-CFTR human airway epithelia. The FASEB Journal. Retrieved from https://www.fasebj.org/content/28/2/791.short

Brayden, D., & Walsh, E. (2014). Efficacious Intestinal Permeation Enhancement Induced by the Sodium Salt of 10-undecylenic Acid, A Medium Chain Fatty Acid Derivative. The AAPS journal. Retrieved from https://link.springer.com/article/10.1208/s12248-014-9634-3

Collingwood, S., Howsham, C., & Hunt, T. (2014). 3, 5-diamino-6-chloro-pyrazine-2-carboxylic acid derivatives and their use as epithelial sodium channel blockers for the treatment of airway diseases. US Patent 8,664,228. Retrieved from https://www.google.com/patents/US8664228

Hubbard, D., Ghandehari, H., & Brayden, D. (2014). Transepithelial transport of PAMAM dendrimers across isolated rat jejunal mucosae in Ussing chambers. Biomacromolecules. Retrieved from https://pubs.acs.org/doi/abs/10.1021/bm5004465

Jung, E., Park, J., Gee, H., & Jung, J. (2014). Shank2 mutant mice display a hypersecretory response to cholera toxin. The Journal of  …. Retrieved from https://jp.physoc.org/content/592/8/1809.short

Ko, E., Jin, B., Namkung, W., & Ma, T. (2014). Chloride channel inhibition by a red wine extract and a synthetic small molecule prevents rotaviral secretory diarrhoea in neonatal mice. Gut. Retrieved from https://gut.bmj.com/content/63/7/1120.short

Kosik-Bogacka, D., & Mlodzik-Danielewicz, N. (2014). Effect of amiloride and bumetanide on transepithelial ion transport in isolated rabbit cecal and colonic wall. Journal cover. Retrieved from https://www.phmd.pl/fulltxthtml.php?ICID=16306

Lomasney, K. (2014). Converging effects of a Bifidobacterium and Lactobacillus probiotic strain on mouse intestinal physiology. American journal of  …. Retrieved from https://ajpgi.physiology.org/content/ajpgi/early/2014/05/21/ajpgi.00401.2013.full.pdf

Lomasney, K., & Houston, A. (2014). Selective influence of host microbiota on cAMP-mediated ion transport in mouse colon. …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1111/nmo.12328/full

Markov, A., & Falchuk, E. (2014). Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins. … -European Journal of  …. Retrieved from https://link.springer.com/article/10.1007/s00424-014-1460-z

Suntornsaratoon, P. (2014). Pre-suckling calcium supplementation effectively prevents lactation-induced osteopenia in rats. American Journal of …. Retrieved from https://ajpendo.physiology.org/content/306/2/E177.short

Tradtrantip, L., Ko, E., & Verkman, A. (2014). Antidiarrheal Efficacy and Cellular Mechanisms of a Thai Herbal Remedy. PLoS neglected tropical diseases. Retrieved from https://dx.plos.org/10.1371/journal.pntd.0002674.g007

Wang, Y., Tong, J., & Chang, B. (2014). Effects of alcohol on intestinal epithelial barrier permeability and expression of tight junction-associated proteins. Molecular medicine  …. Retrieved from https://www.ingentaconnect.com/content/sp/mmr/2014/00000009/00000006/art00045

Welling, S., Hubálek, F., & Jacobsen, J. (2014). The role of citric acid in oral peptide and protein formulations: Relationship between calcium chelation and proteolysis inhibition. European Journal of  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0939641113003998

Yusef, Y., Thomas, W., & Harvey, B. (2014). Estrogen increases ENaC activity via PKCδ signaling in renal cortical collecting duct cells. Physiological reports. Retrieved from https://physreports.physiology.org/content/2/5/e12020.abstract

Zhou, Y., Chu, W., Lei, M., Li, J., Du, W., & Zhao, C. (2014). Application of a continuous intrinsic dissolution–permeation system for relative bioavailability estimation of polymorphic drugs. International journal of  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S037851731400502X

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