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LWCC-3500

LWCC-3500

500cm Liquid Waveguide Capillary Flow Cell


  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

LWCC Typical LWCC setup includes an injection system, a pump, and a spectrophotometer.

There are 2 images available to view - click to enlarge and scroll through the product gallery.

Data Sheet
/ Download as PDF

  • 1250-uL Internal Sample Volume
  • Improve sensitivity 500 times vs standard quartz type 10-mm cuvettes.
  • Wavelength Range: 280-730nm

Microliter sample volumes — exceptional sensitivity

WPI’s Liquid Waveguide Capillary Cell (LWCC) is a flow cell for absorbance measurements in the ultraviolet, visible and near infra-red ranges. Pathlengths range from 50–500cm, with increasing measuement sensitivity from 50 to 500-fold. The flow cells are fiber coupled and have a very small sample volume ranging from 125mL (50cm pathlength) to 1,250mL (500cm pathlength).

How does it work?

The sample solution is introduced into the LWCC at the liquid input. Light is coupled into the LWCC from a light source via a fiber optic cable. After passing through the LWCC, light is collected with an optical fiber and guided to a detector. The concentration of the sample is determined by measuring its absorbance in the LWCC, similar to a standard UV/VIS spectrometer.

efficiencycurvelwcc3000.jpg

These spectra show the optimal detection limits for LWCCs of varying pathlength.

Advantages of LWCC over standard cuvettes

Ultra-sensitive absorbance measurements can be performed in the UV, VIS, and NIR portion of the light spectrum. Compared with a standard 1cm cuvette, a 1mAU signal is enhanced 100-fold to 100mAU when using an LWCC-3100. LWCC units can be directly connected to a pump, a fluid injection analysis system, or even filled with a syringe.

Detector requirements

Based on fiber optics, the LWCC is designed for use with WPI’s LEDspec (biophotometric detection system), Tidas I, Tidas 100 and Tidas E spectrometer systems. The LWCC can also be interfaced to any CCD, PDA or scanning type optical spectrometer or photodiode detector with fiber optic input capabilities. WPI also offers a range of light sources, such as FO-6000 (VIS/NIR studies) and D4H (UV/VIS) which can be used in conjunction with the LWCC.

Wavelength range

Designed to work in the UV, VIS and NIR, the LWCC's optical performance is strongly dependent on the solvent used in the wavelength of interest. Please note that in aqueous solutions the wavelength performance is limited (see Efficiency Curves).

  Pathlength [cm]
Internal Volume [µL]
Wavelength Range [nm]
measured with Tidas II
LWCC-3050 50 125 230-800
LWCC-3100 100 250 230-730
LWCC-3250 250 625 250-730
LWCC-3500 500 1250 280-730

Linearity

By Beer’s Law, the absorption of a liquid sample in LWCC bears a linear relationship to the concentration of an analyte. A linear relationship is observed between 0.01–2AU and is limited only by stray light and noise from the spectrometer.

Chemical resistance

Any chemicals that could react with PEEK, Polyimide and fused silica should not be used in LWCC. (If in doubt, please contact WPI for details.)

Applications

Applications include liquid chromatography detection, stopped-flow injection, flow-injection analysis, gas-segmented continuous flow analysis and water monitoring (environmental, oceanic, and drinking water). Please contact WPI to discuss your needs.

Specifications

  LWCC-3050 LWCC-3100 LWCC-3250 LWCC-3500
Optical Pathlength 50cm 100cm 250cm 500cm
Internal Volume 125µL 250µL 625µL 1250µL
Fiber Connection  500µm SMA  500µm SMA  500µm SMA  500µm SMA
Transmission @254nm* >20 >10 >1 -
Transmission @540nm* >35 >30 >30 20
Noise [mAU]** < 0.1 < 0.2   < 1.0
Maximum Pressure 100 PSI 100 PSI 100 PSI 100 PSI
Wetted Material PEEK, Fused Silica, PTFE PEEK, Fused Silica, PTFE PEEK, Fused Silica, PTFE PEEK, Fused Silica, PTFE
Liquid Input Standard 10-32 Coned Port Fitting Standard 10-32 Coned Port Fitting Standard 10-32 Coned Port Fitting Standard 10-32 Coned Port Fitting

* Referenced using coupled 500µm fibers        
** Measured using ASTM E685-93            
*** A one-meter waveguide of 550µm internal diameter requires approximately 1.5PSI for water flow of 1.0mL/min.

 

foefficiency_color.jpg

 

When comparing light throughput versus wavelength of three fiber optic cables, the greater the diameter of the cable, the better the LWCC performance up to 500µm which is the input diameter of the SMA connector.

Accessories

89372

89372

LWCC Injection System

View details...

Citations

Bonifay, V., Barrett, T., & Pattison, D. (2014). Tryptophan oxidation in proteins exposed to thiocyanate-derived oxidants. Archives of biochemistry  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0003986114003142

Catelani, T., Tóth, I., Lima, J., Pezza, L., & Pezza, H. (2014). A simple and rapid screening method for sulfonamides in honey using a flow injection system coupled to a liquid waveguide capillary cell. Talanta. Retrieved from https://www.sciencedirect.com/science/article/pii/S0039914013010163

Chaparro, L., Ferrer, L., Leal, L., & Cerdà, V. (2014). A multisyringe flow-based system for kinetic–catalytic determination of cobalt (II). Talanta. Retrieved from https://www.sciencedirect.com/science/article/pii/S0039914014005360

Du, Z., He, K., Cheng, Y., Duan, F., & Ma, Y. (2014). A yearlong study of water-soluble organic carbon in Beijing II: Light absorption properties. Atmospheric  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S1352231014001186

Fang, T., Verma, V., & Guo, H. (2014). A semi-automated system for quantifying the oxidative potential of ambient particles in aqueous extracts using the dithiothreitol (DTT) assay: results from the. Atmospheric  …. Retrieved from https://www.atmos-meas-tech-discuss.net/7/7245/2014/

Gil-Lozano, C., & Losa-Adams, E. (2014). Pyrite nanoparticles as a Fenton-like reagent for in situ remediation of organic pollutants. Beilstein journal of  …. Retrieved from https://www.beilstein-journals.org/bjnano/content/5/1/97

Hopwood, M., Statham, P., & Milani, A. (2014). Dissolved Fe (II) in a river-estuary system rich in dissolved organic matter. Estuarine, Coastal and Shelf Science. Retrieved from https://www.sciencedirect.com/science/article/pii/S0272771414002674

Kolacinska, K., & Trojanowicz, M. (2014). Application of flow analysis in determination of selected radionuclides. Talanta. Retrieved from https://www.sciencedirect.com/science/article/pii/S0039914014001556

Kröckel, L., Lehmann, H., Wieduwilt, T., & Schmidt, M. (2014). Fluorescence detection for phosphate monitoring using reverse injection analysis. Talanta. Retrieved from https://www.sciencedirect.com/science/article/pii/S0039914014001702

Lee, S., Cha, W., Kim, J., Baik, M., & Jung, E. (2014). Uranium (IV) remobilization under sulfate reducing conditions. Chemical  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0009254114000618

Ma, J., Adornato, L., Byrne, R., & Yuan, D. (2014). Determination of nanomolar levels of nutrients in seawater. TrAC Trends in Analytical Chemistry. Retrieved from https://www.sciencedirect.com/science/article/pii/S0165993614001095

Maugendre, L., & Gattuso, J. (2014). Effect of ocean warming and acidification on a plankton community in the NW Mediterranean Sea. ICES Journal of  …. Retrieved from https://icesjms.oxfordjournals.org/content/early/2014/09/24/icesjms.fsu161.short

Milani, A. (2014). Development of microfluidic technology for in-situ determination of iron and manganese in natural aquatic systems. Retrieved from https://eprints.soton.ac.uk/365471/

Moreno, D. V., & Manchado, P. (n.d.). Optical phytoplankton discriminator (OPD) developed for a gliper. upcommons.upc.edu. Retrieved from https://upcommons.upc.edu/revistes/handle/2099/14693

Ogawa, H., Kogure, K., & Kanda, J. (2014). Detailed Variations in Bioactive Elements in the Surface Ocean and Their Interaction with Microbiological Processes. Retrieved from https://www.terrapub.co.jp/e-library/w-pass/pdf/w-pass_177.pdf

Powers, L., & Miller, W. (2014). Blending remote sensing data products to estimate photochemical production of hydrogen peroxide and superoxide in the surface ocean. Environmental Science: Processes & Impacts. Retrieved from https://pubs.rsc.org/EN/content/articlehtml/2014/em/c3em00617d

Pulido-Villena, E. (2014). Microbial food web dynamics in response to a Saharan dust event: results from a mesocosm study in the oligotrophic Mediterranean Sea. Biogeosciences  …. Retrieved from https://www.biogeosciences-discuss.net/11/337/2014/bgd-11-337-2014.html

Ridame, C., & Dekaezemacker, J. (2014). Contrasted Saharan dust events in LNLC environments: impact on nutrient dynamics and primary production. …. Retrieved from https://www.biogeosciences.net/11/4783/2014/bg-11-4783-2014.html

Srinivas, B., & Sarin, M. (2014). Brown carbon in atmospheric outflow from the Indo-Gangetic Plain: Mass absorption efficiency and temporal variability. Atmospheric Environment. Retrieved from https://www.sciencedirect.com/science/article/pii/S1352231014002015

Srinivas, B., Sarin, M., & Sarma, V. (2014). Atmospheric outflow of nutrients to the Bay of Bengal: Impact of anthropogenic sources. Journal of Marine Systems. Retrieved from https://www.sciencedirect.com/science/article/pii/S0924796314001742

Sunscreens as a Source of Hydrogen Peroxide Production in Coastal Waters. (2014).Environmental science &  …. Retrieved from https://pubs.acs.org/doi/abs/10.1021/es5020696

Uthuppu, B. (2014). Polymer coated gold nanoparticles for tracing the mobility of engineered nanoparticles in the subsurface. European  …. Retrieved from https://meetingorganizer.copernicus.org/EGU2014/EGU2014-5719.pdf

Yuan, X., Miller, C., Pham, A., & Waite, T. (2014). Kinetics and mechanism of auto-and copper-catalyzed oxidation of 1, 4-naphthohydroquinone. Free Radical Biology and  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0891584914001385

Zhu, Y., Yuan, D., Huang, Y., Ma, J., Feng, S., & Lin, K. (2014). A modified method for on-line determination of trace ammonium in seawater with a long-path liquid waveguide capillary cell and spectrophotometric detection. Marine Chemistry. Retrieved from https://www.sciencedirect.com/science/article/pii/S0304420314000620

 

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