NL2010MC4 - Discontinued

Overview

Discontinued, see Nanoliter2020

• Microprocessor-controlled injector
• Uses direct piston displacement
• Filling and injection speeds: 23nL/sec and 46 nL/sec
• Optional controller or footswitch

Injection volumes

WPI's microprocessor-controlled Nanoliter 2010 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/sec). Maximum fluid ejection is 5 µL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment.

Included with pump

Included: 1 vial 3.5 in. capillaries (300); replacement "O" rings; Allen wrench; MicroFil™ MF34G backfilling needle; and two sample pre-pulled micropipettes.

Smart controller

MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display. From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start/stop operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.

Specifications

INJECTION VOLUME	Variable in discreet aliquot volumes
REMOTE CONTROL	Yes
GLASS OD	1.14mm
GLASS ID	0.5mm
STEP	12.7µm/step
INJECTION SPEED: Slow	23nL/sec
INJECTION SPEED: Fast	46nL/sec
FILL SPEED: Slow	23nL/sec
FILL SPEED: Fast	46nL/sec
EMPTY SPEED	92nL/sec
VARIABLE VOLUME RANGE	2.3 - 69.0nL
SMALLEST VOLUME	2.3nL
INJECTIONS PER FILLING, MAX.	100 injections
SHIPPING WEIGHT	3 lb. (1.1 kg)

Accessories

Citations

Bawin, T., & Boukraa, S. (2014). Using micro-injection technique to assess fungal toxicity in mosquito control. …  in Agricultural and …. Retrieved from https://orbi.ulg.ac.be/handle/2268/162921

Eom, K., Kim, J., Choi, J., Kang, T., & Chang, J. (2014). Enhanced Infrared Neural Stimulation using Localized Surface Plasmon Resonance of Gold Nanorods. Small. Retrieved from https://onlinelibrary.wiley.com/doi/10.1002/smll.201400599/full

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