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Manual Electrode for EVOM2 for use with Millipore Multiscreen HTS 96-Well Plate

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STX100 Instruction Manual
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  • Use with Millipore Multiscreen HTS 96-Well Plate
  • Improved accuracy down to 5 Ohm
  • Sterilize with EtO, alcohol, or bactericide

Use in HTS

With the development of a High Throughput Screening (HTS) protocol for faster drug discovery, a new line of cell culture filter plates have been introduced by several major cell culture insert manufacturers. These HTS plates normally have either 24 or 96 individual cell culture inserts “bonded” together as one plate so that it can be handled by a robot apparatus. In response to these developments, WPI has developed an automatic REMS system and a manual electrode, STX100, for TEER measurements using HTS plates.

Increase accuracy of TEER measurements

STX100’s design is based on the same reliable design principle as the universally used STX2 electrode, with several important modifications. The size of the electrode tip has been reduced to 1.5 mm to facilitate positioning through the narrower slit of the HTS plate. The STX100 electrode itself is constructed using a stronger material for higher durability and maximum usage applications. The bottom section of the electrode is shaped to fit neatly into the “keyhole” shaped filter well. This enables the STX100 electrode to produce increased accuracy and reproducibility of TEER readings (±5 ohms) compared to the standard STX2. Measurement can be directly performed when the HTS plate is in either a common or divided tray, reducing the possibility of contamination as well as mechanical damage to the cultured cells.

The STX100M is compatible with the following Millpore plates:

PN Description Pore Size Membrane
PSHT004R1 96-well plate 0.4um PCF
PSRP004R1 96-well plate 1.0um PET
PSHT004R5 96-well plate 0.4um PCF
PSRP004R5 96-well plate 1.0um PET
PSHT004S5 96-well plate 0.4um PCF





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