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Dr Gary Lawrence, Dublin City University

WPI are my preferred supplier for tissue and cell recording instrumentation. In my experience, WPI representatives have an excellent level of technical expertise and they have always responded in a timely manner with the most helpful, patient and instructive advice to aid the development of the o...
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BT-50MM-01

BT-50MM-01

BiOTESTER: A fully equipped biaxial test system built specifically for biomaterials


  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

BIOTESTER Biorakes with tissue mounted on a BioTester. The sample size is much smaller than a US penny One view from the software with actual live video from the mounted sample. Biorakes Pulley System Clamps

There are 6 images available to view - click to enlarge and scroll through the product gallery.

Biotester Datasheet
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Uniaxial U-Stretch
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Why measure mechanical properties?
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Stress-strain in human sclera
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The BioTester provides researchers with an easy-to-use, affordable test instrument to characterize soft tissues and biomaterials. This biaxial test system captures and graphically displays live time, force, and synchronized video images for results analysis and verification. Data is easily exported to standard spreadsheet programs. 

  • High performance actuators (2 per axis) capable of μm positional resolution for accurate test motion.
  • Inline overload-protected load cell on each axis
  • High resolution CCD camera to collect time synchronized images for post test analysis
  • Temperature controlled media bath
  • Patented attachment system facilitates rapid and accurate specimen attachment
  • Optional use of hook-and-suture or grip based attachment systems
  • User-controlled test routines for multi-modal cyclic, simple, and relaxation testing over a wide range of strain rates
  • Data output as a comma separated value text files for easy import into a variety of spread sheet and data analysis programs
  • Simple USB connection to a Windows-based host computer
  • Specimen Size: 3mm to 15mm
  • Load Cell Capacities (N): 0.5, 1.5, 2.5, 5, 10, 23
  • Load Cell Accuracies: 0.2% of capacity Max Displacement
  • Rate: 10mm/s Image Rate: 15Hz
  • Image Resolution: 1280 X 960 pixels
  • Max. Temperature: 40°C

Sample Mounting Systems

The BioRake sample mounting system is CellScale’s patented method for attaching soft tissues and biomaterials.

 Each tine is electrochemically sharpened to easily pierce both the toughest and most delicate tissue samples. Each set is permanently attached to a common base to allow simultaneous puncture of all 20 attachment points. The BioRakes are magnetically mounted for easy removal for cleaning or replacement and for simple transition between BioRake, Balanced Pulley, and Clamp mounting systems.

To perform testing, samples are positioned and raised into place using the manual lift mechanism and pressure is applied to insert the hooks in the tissue. The sample is thus mounted and ready for analysis within a few seconds. The mounting is consistent, accurate and easy.

BioRakes are available with tine spacing ranging from 0.7mm to 2.2mm to accommodate specimens from 3 to 15mm in size.

The balanced pulley sample mounting system is CellScale’s attachment method for ensuring zero shear stress during biaxial testing.

 

Two double-ended custom suture hooks are used to create 4 attachment points on each side of the specimen. A two-stage stainless steel pulley mechanism ensures that each of the sutures is held at the same tension during the test.

The pulley mechanisms are magnetically mounted for easy removal for cleaning and for simple transition between BioRake, Balanced Pulley, and Clamp mounting systems.

The clamp sample mounting system is CellScale’s attachment method for testing to failure.

 

Using a cruciform specimen allows the attachment sites, which are inherently weaker than the base material, to be moved away from the gauge area of the specimen. The clamps allow the specimen to be loaded easily and held securely.

The stainless steel clamping mechanisms are mounted over the same brackets used for the other attachment systems to allow for fast and easy transition between BioRake, Balanced Pulley, and Clamp mounting systems.

Custom clamp designs can also be made to tailor the clamp force and clamping surface to your tissue. 

Video Overviews 

 

Specifications

Force Capacity  500, 100, 2500, 5000mN, 10N, 23N
Force Accuracy  0.2% of force capacity
Max. Elongation Rate 10mm/s
Max. Strain Rate (5mm specimen) 200%/s
Spatial Resolution (Actuator)  >0.1μm 
Spatial Accuracy (Acuator) 10μm 
Spatial Resolution (Image Analysis)  1/8 pixel 
Max. Force Data Rate 100Hz
Image Rate 1280 x 960 -15Hz

Accessories

Citations

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Laurence, Devin & Homburg, Hannah & Tang, Qinggong & Fung, Kar-Ming & Bohnstedt, Bradley & Holzapfel, Gerhard & Lee, Chung-Hao. (2021). A pilot study on biaxial mechanical, collagen microstructural, and morphological characterizations of a resected human intracranial aneurysm tissue. Scientific Reports. 11. 10.1038/s41598-021-82991-x.

Leonov, D. & Spirina, Yu & Yatsenko, A. & Kushnarev, Vladimir & Ustinov, E. & Barannikov, S. (2021). Advanced 3D Bioprinting Technologies. Cell and Tissue Biology. 15. 616-627. 10.1134/S1990519X21060134.

Borem, Ryan & Madeline, Allison & Theos, Chris & Vela, Ricardo & Garon, Alex & Gill, Sanjitpal & Mercuri, Jeremy. (2021). Angle-ply scaffold supports annulus fibrosus matrix expression and remodeling by mesenchymal stromal and annulus fibrosus cells. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 10.1002/jbm.b.34980.

McClarty, Davis & Ouzounian, Maral & Tang, Mingyi & Eliathamby, Daniella & Romero, David & Nguyen, Elsie & Simmons, Craig & Amon, Cristina & Chung, Jennifer. (2021). Ascending aortic aneurysm haemodynamics are associated with aortic wall biomechanical properties.  European Journal of Cardio-Thoracic Surgery. 10.1093/ejcts/ezab471.

Ndlovu, Zwelihle & Desai, Dawood & Pandelani, Thanyani & Ngwangwa, Harry & Nemavhola, Fulufhelo. (2021). Biaxial estimation of biomechanical constitutive parameters of passive porcine sclera soft tissue.  10.31224/osf.io/h27wp.

Filippo Valente, Matt S. Hepburn, Jingyu Chen, Ana A. Aldana, Benjamin J. Allardyce, Sajjad Shafei, Barry J. Doyle, Brendan F. Kennedy, Rodney J. Dilley,  (2021). Bioprinting silk fibroin using two-photon lithography enables control over the physico-chemical material properties and cellular response. Bioprinting. 25. e00183. 10.1016/j.bprint.2021.e00183.

Lomboni, David & Steeves, Alexander & Schock, Sarah & Bonetti, Lorenzo & De Nardo, Luigi & Variola, Fabio. (2021). Compounded topographical and physicochemical cueing by micro-engineered chitosan substrates on rat dorsal root ganglion neurons and human mesenchymal stem cells. Soft Matter. 17. 10.1039/D0SM02170A.

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Nemavhola, Fulufhelo & Ngwangwa, Harry & Pandelani, Thanyani. (2021). Experimental analysis and biaxial biomechanical behaviour of ex-vivo sheep trachea. 10.1101/2021.11.26.470180.

Durbak, Emily & Tarraf, Samar & Gillespie, Callan & Germano, Emídio & Cikach, Frank & Blackstone, Eugene & Emerton, Kelly & Colbrunn, Robb & Bellini, Chiara & Roselli, Eric. (2021). Ex-vivo Biaxial Load Testing Analysis of Aortic Biomechanics Demonstrates Variation in Elastic Energy Distribution Across the Aortic Zone Zero. The Journal of Thoracic and Cardiovascular Surgery. 10.1016/j.jtcvs.2021.09.071.

Nemavhola, Fulufhelo & Pandelani, Thanyani & Ngwangwa, Harry. (2021). Fitting Of Hyperelastic Constitutive Models In Different Sheep Heart Regions Based On Biaxial Mechanical Properties. 10.1101/2021.10.28.466240.

Ramburrun, Poornima & Kumar, Pradeep & Ndobe, Elias & Choonara, Yahya. (2021). Gellan-Xanthan Hydrogel Conduits with Intraluminal Electrospun Nanofibers as Physical, Chemical and Therapeutic Cues for Peripheral Nerve Repair. International Journal of Molecular Sciences. 22. 11555. 10.3390/ijms222111555.

Hudson, Luke & Laurence, Devin & Lau, Hunter & Mullins, Brennan & Doan, Deenna & Lee, Chung-Hao. (2021). Linking collagen fiber architecture to tissue-level biaxial mechanical behaviors of porcine semilunar heart valve cusps. Journal of the Mechanical Behavior of Biomedical Materials. 125. 104907. 10.1016/j.jmbbm.2021.104907.

Surman, Tim & O'Rourke, Dermot & Reynolds, Karen & Edwards, J. & Worthington, M. (2021). M06 The Unique Tissue Biomechanics of the Thoracic Aorta. What are the Greatest Areas of Weakness and Where Should we Focus Repair?. Heart, Lung and Circulation. 30. S4. 10.1016/j.hlc.2021.03.015.

Singh, B. & Singh, G. & Chard, R. & Nicholson, I. (2021). M08 Perioperative and Midterm Outcomes of Aortic Root Enlargements Compared to Conventional and Rapid Deployment Prosthesis. Heart, Lung and Circulation. 30. S4-S5. 10.1016/j.hlc.2021.03.017.

Walsh, Darragh & Ross, Aisling & Newport, David & Zhou, Zhou & Kearns, Jamie & Fearon, Conor & Lorigan, Jennifer & Mulvihill, John. (2021). Mechanical Characterisation of the Human Dura Mater, Falx Cerebri and Superior Sagittal Sinus. Acta Biomaterialia. 134. 10.1016/j.actbio.2021.07.043.

Cunnane, Eoghan & Davis, NIALL & Cunnane, Connor & Lorentz, Katherine & Ryan, Alan & Hess, Jochen & Weinbaum, Justin & Walsh, Michael & O’Brien, Fergal & Vorp, David. (2021). Mechanical, compositional and morphological characterisation of the human male urethra for the development of a biomimetic tissue engineered urethral scaffold. Biomaterials. 269. 120651. 10.1016/j.biomaterials.2021.120651.

Maleckis, Kaspars & Kamenskiy, Alexey & Lichter, Eliezer & Oberley-Deegan, Rebecca & Dzenis, Yuris & Mactaggart, Jason. (2021). Mechanically Tuned Vascular Graft Demonstrates Rapid Endothelialization and Integration Into the Porcine Iliac Artery Wall. Acta Biomaterialia. 125. 10.1016/j.actbio.2021.01.047.

Morningstar, Jordan & Gensemer, Cortney & Moore, Reece & Fulmer, Diana & Beck, Tyler & Wang, Christina & Moore, Kelsey & Guo, Lilong & Sieg, Franz & Nagata, Yasufumi & Bertrand, Philippe & Spampinato, Ricardo & Glover, Janiece & Poelzing, Stephen & Gourdie, Robert & Watts, Kelsey & Richardson, William & Levine, Robert & Borger, Michael & Norris, Russell. (2021). Mitral Valve Prolapse Induces Regionalized Myocardial Fibrosis. Journal of the American Heart Association. 10. 10.1161/JAHA.121.022332.

Ahmad, Dilshad & Ajaj, Rafic. (2021). Multiaxial Mechanical Characterization of Latex Skin for Morphing Wing Application. Polymer Testing. 10.1016/j.polymertesting.2021.107408.

Zheng, Cheng & Ding, Kailei & Huang, Xueyu & Li, Meiling & Wu, Bingang & Lei, Yang & Wang, Yunbing. (2021). Nonglutaraldehyde crosslinked bioprosthetic heart valves based on 2-isocyanatoethyl methacrylate crosslinked porcine pericardium with improved properties of stability, cytocompatibility and anti-calcification. Composites Part B: Engineering. 230. 109504. 10.1016/j.compositesb.2021.109504.

Nemavhola, Fulufhelo & Ngwangwa, Harry & Davies, Neil & Franz, Thoams. (2021). Passive Biaxial Tensile Dataset of Three Main Rat Heart Myocardia: Left Ventricle, Mid-Wall and Right Ventricle. 10.20944/preprints202108.0153.v1.

Liu, Hailong & Jain, Shubham & Ahlinder, Astrid & Fuoco, Tiziana & Gasser, Thomas & Finne-Wistrand, Anna. (2021). Pliable, Scalable, and Degradable Scaffolds with Varying Spatial Stiffness and Tunable Compressive Modulus Produced by Adopting a Modular Design Strategy at the Macrolevel. ACS Polymers Au. XXXX. 10.1021/acspolymersau.1c00013.

Ross, Colton & Hsu, Ming-Chen & Baumwart, Ryan & Mir, Arshid & Burkhart, Harold & Holzapfel, Gerhard & Wu, Yi & Lee, Chung-Hao. (2021). Quantification of load-dependent changes in the collagen fiber architecture for the strut chordae tendineae-leaflet insertion of porcine atrioventricular heart valves. Biomechanics and Modeling in Mechanobiology. 20. 10.1007/s10237-020-01379-4.

Ndlovu, Zwelihle & Desia, Dawood & Nemavhola, Fulufhelo & Ngwangwa, Harry. (2021). Sheep Sclera Soft Tissue Subjected to Mechanical Equi-Biaxial Testing. 10.20944/preprints202108.0388.v1.

Quince, Zachery & Alonso-Caneiro, David & Read, Scott & Collins, Michael. (2021). Static compression optical coherence elastography to measure soft contact lens mechanical properties. Biomedical Optics Express. 12. 10.1364/BOE.419344.

Nemavhola, Fulufhelo. (2021). Study of biaxial mechanical properties of the passive pig heart: material characterisation and categorisation of regional differences. International Journal of Mechanical and Materials Engineering. 16. 10.1186/s40712-021-00128-4.

Hu, Yingbing & Huang, Yu & Chen, Yun & Ye, Cheng & Wei, Wei & Feng, Yun & Mi, Shengli. (2021). Study on patterned photodynamic cross-linking for keratoconus. Experimental Eye Research. 204. 108450. 10.1016/j.exer.2021.108450.

Cai, Li & Zhang, Ruihang & Li, Yiqiang & Guangyu, Zhu & Ma, Xingshuang & Wang, Yongheng & Luo, Xiaoyu & Gao, Hao. (2021). The Comparison of Different Constitutive Laws and Fiber Architectures for the Aortic Valve on Fluid–Structure Interaction Simulation. Frontiers in Physiology. 12. 682893. 10.3389/fphys.2021.682893.

Meador, William & Zhou, Jennifer & Malinowski, Marcin & Jazwiec, Tomasz & Calve, Sarah & Timek, Tomasz & Rausch, Manuel. (2021). The effects of a simple optical clearing protocol on the mechanics of collagenous soft tissue. Journal of Biomechanics. 122. 110413. 10.1016/j.jbiomech.2021.110413.

Barrett, Jeff & Fewster, Kayla & Cudlip, Alan & Dickerson, Clark & Callaghan, Jack. (2021). The rate of tendon failure in a collagen fibre recruitment-based model. Journal of the Mechanical Behavior of Biomedical Materials. 115. 104273. 10.1016/j.jmbbm.2020.104273.

Sawadkar, Prasad & Nandin, Mandakhbayar & Patel, Kapil & Buitrago, Jennifer & Kim, Tae & Rajasekar, Poojitha & Lali, Ferdinand & Kyriakidis, Christos & Rahmani, Benyamin & Mohanakrishnan, Jeviya & Dua, Rishbha & Greco, Karin & Lee, Jung-Hwan & Kim, Hae-Won & Knowles, Jonathan & García-Gareta, Elena. (2021). Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration. Journal of Tissue Engineering. 12. 1-17. 10.1177/20417314211019238.

Nemavhola, Fulufhelo & Ngwangwa, Harry & Pandelani, Thanyani & Davies, Neil & Franz, Thomas. (2021). Understanding regional mechanics of rat myocardia by fitting hyperelatsic models. 10.21203/rs.3.rs-957393/v1.

Yang, Fan & Xu, Liangpeng & Guo, Gaoyang & Wang, Yunbing. (2021). Visible light–induced cross-linking of porcine pericardium for the improvement of endothelialization, anti-tearing, and anticalcification properties. Journal of Biomedical Materials Research Part A. 110. 10.1002/jbm.a.37263.

Lan, Xiaorong & Zhao, Qianting & Zhang, Jiayi & Lei, Yang & Wang, Yunbing. (2020). A combination of hydrogen bonding and chemical covalent crosslinking to fabricate a novel swim-bladder-derived dry heart valve material yields advantageous mechanical and biological properties. Biomedical Materials. 16. 10.1088/1748-605X/abb616.

A. Cudlip (2020). A combined in vivo and in vitro approach to assess supraspinatus activation and tissue responses to arm elevation demands

Sang, Cyril & Kallmes, D. & Kadirvel, R. & Durka, Micheal & Ding, Y-H & Dai, D. & Watkins, S. & Robertson, Anne (2020). Adaptive Remodeling in the Elastase-Induced Rabbit Aneurysms. Experimental Mechanics. 61. 10.1007/s11340-020-00671-9.

Sharifi Kia, Danial & Benza, Evan & Bachman, Timothy & Tushak, Claire & Kim, Kang & Simon, Marc. (2020). Angiotensin Receptor-Neprilysin Inhibition Attenuates Right Ventricular Remodeling in Pulmonary Hypertension. Journal of the American Heart Association. 9. 10.1161/JAHA.119.015708.

Schaefer, Douglas & Khan, Sehroon & Nadir, Sadia & Dong, Yang & Mortimer, Peter & Gui, Heng & Khan, Afsar & Yu, Mingming & Iqbal, Shahid & Sheng, Jun & Xu, Jianchu. (2020). Biodegradation of polyester polyurethane by Aspergillus flavus G10. 10.1101/2020.06.25.170654.

Chung, Jennifer & Wong, Edwin & Tang, Mingyi & Eliathamby, Daniella & Forbes, Thomas & Butany, Jagdish & Simmons, Craig & Ouzounian, Maral. (2020). Biomechanics of Aortic Dissection: A Comparison of Aortas Associated With Bicuspid and Tricuspid Aortic Valves. Journal of the American Heart Association. 9. 10.1161/JAHA.120.016715.

Ahn, Seungkuk & Chantre, Christophe & Ardona, Herdeline Ann & Gonzalez, Grant & Campbell, Patrick & Parker, Kevin. (2020). Biomimetic and estrogenic fibers promote tissue repair in mice and human skin via estrogen receptor β. Biomaterials. 255. 120149. 10.1016/j.biomaterials.2020.120149.

Virgilio, Kelley & Jones, Brian & Miller, Emily & Ghajar-Rahimi, Elnaz & Martin, Kyle & Peirce, Shayn & Blemker, Silvia. (2020). Computational Models Provide Insight into In Vivo Studies and Reveal the Complex Role of Fibrosis in mdx Muscle Regeneration. Annals of Biomedical Engineering. 49. 10.1007/s10439-020-02566-1.

Jadidi, Majid & Sherifova, Selda & Sommer, Gerhard & Kamenskiy, Alexey & Holzapfel, Gerhard. (2020). Constitutive modeling using structural information on collagen fiber direction and dispersion in human superficial femoral artery specimens of different ages. Acta Biomaterialia. 121. 10.1016/j.actbio.2020.11.046.

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Whittal, Mitchel & Molladavoodi, Sara & Zwambag, Derek & Millecamps, Magali & Stone, Laura & Gregory, Diane. (2020). Mechanical Consequence of Induced Intervertebral Disc Degeneration in the SPARC-Null Mouse. Journal of Biomechanical Engineering. 143. 10.1115/1.4047995.

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Yang, Fan & Xu, Liangpeng & Kuang, Dajun & Ge, Yao & Guo, Gaoyang & Wang, Yunbing. (2020). Polyzwitterion-crosslinked hybrid tissue with antithrombogenicity, endothelialization, anticalcification properties. Chemical Engineering Journal. 410. 128244. 10.1016/j.cej.2020.128244.

Yang, Li & Huang, Xueyu & Deng, Lu & Ma, Xiaoxiao & Jiang, Honglin & Ning, Qinggong & Liang, Zhen & Lei, Yang & Wang, Yunbing. (2020). Pre-mounted dry TAVI valve with improved endothelialization potential using REDV-loaded PEGMA hydrogel hybrid pericardium. Journal of Materials Chemistry B. 8. 10.1039/C9TB00879A.

Aldosary, Ghada & Tse, Tabitha & Arnaout, Angel & Caudrelier, Jean-Michel & Czyrnyj, Catriona & Romain, Ron & Mclean, Linda & Foottit, Claire & Vandervoort, Eric & Belec, Jason. (2020). Radiological, dosimetric and mechanical properties of a deformable breast phantom for radiation therapy and surgical applications. Biomedical Physics & Engineering Express. 6. 10.1088/2057-1976/ab834a.

Barbour, Kaitlyn & Huang, Hsiao-Ying. (2020). Strain effects on collagen proteolysis in heart valve tissues. Mechanics of Time-Dependent Materials. 24. 10.1007/s11043-019-09410-7.

Yang, Li & Xie, Shuang & Ding, Kailei & Lei, Yang & Wang, Yunbing. (2020). The study of dry biological valve crosslinked with a combination of carbodiimide and polyphenol. Regenerative Biomaterials. 8. 10.1093/rb/rbaa049.

Meador, William & Mathur, Mrudang & Sugerman, Gabriella & Malinowski, Marcin & Jazwiec, Tomasz & Wang, Xinmei & Lacerda, Carla & Timek, Tomasz & Rausch, Manuel. (2020). The tricuspid valve also maladapts as shown in sheep with biventricular heart failure. eLife. 9. 10.7554/eLife.63855.

Meador, William & Mathur, Mrudang & Sugerman, Gabriella & Malinowski, Marcin & Jazwiec, Tomasz & Wang, Xinmei & Lacerda, Carla & Timek, Tomasz & Rausch, Manuel. (2020). The tricuspid valve also maladapts: A multiscale study in sheep with biventricular heart failure. 10.1101/2020.09.03.278515.

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