World Precision Instruments

Search the site by KEYWORD


Quick Finder


Testimonials

Bill Franks, University of Liverpool

With such a good knowledge base of their products and field of operation I know that there is always a quick answer at the end of a phone.
read more

FD35-100

FD35-100

FluoroDish Cell Culture Dish - 35mm, box of 100


Choose Quantity
  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

FD35-100

There are 1 images available to view - click to enlarge and scroll through the product gallery.

FluoroDish Datasheet
/ Download as PDF

FluoroDish Assay
/ Download as PDF

FluoroDish Certification
/ Download as PDF

FluoroDish Certification 2
/ Download as PDF

  • Optical quality glass bottom for better imaging quality
  • Low sample volume for expensive chemicals
  • Lowest access angle for micropipette
  • Low toxicity adhesive for embryo research

Measurements

ID: 33mm * OD: 35.5mm * Glass Diameter Φ: 23.5mm * height (inside): 7.8mm * Height (outside): 9mm * Access angle: 29 degrees

FluoroDish Schematic 

Exceptional Imaging Quality

WPI’s FluoroDish™ tissue culture dishes are now available in a larger range of sizes and coatings. These polycarbonate dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish, substantially better than some other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness stays within the 0.17 ±0.01 mm range.

Excellent for both classical and fluorescence microscopy

Each WPI dish has a flat (0.17 mm thick) optical quality glass bottom, allowing the use of a short objective working distance, large numerical aperture (NA), and a high magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in Image Analysis. The glass bottom permits the use of immersion objectives with medium such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.

Materials

An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. All WPI dishes have the advantages of low toxicity and good UV transmission bottom glass. They are individually packed and gamma sterilized.

Features

The 35 mm dish has outside dimensions similar to that of a Corning 35 mm dish and has ø23.5 mm glass window. Most heaters and perfusion adapters designed for the Corning 35 mm dish will also fit this dish. Certain types of cell lines (e.g., PC3 and HEK) adhere well to the uncoated glass bottom dish. The users can also apply to the uncoated dish any special coating that is best for their cell line.

Human neural stem cells freshly dispersed by Accutase seeded on the glass coverslip bottom of a WPI Fluorodish, timelapse-imaged over a period of 6 days.

Specifications

Accessories

Citations

Addis, R., Ifkovits, J., & Pinto, F. (2013). Optimization of Direct Fibroblast Reprogramming to Cardiomyocytes Using Calcium Activity as a Functional Measure of Success. Journal of molecular and …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0022282813001351

Badique, F., Stamov, D., & Davidson, P. (2013). Directing nuclear deformation on micropillared surfaces by substrate geometry and cytoskeleton organization. Biomaterials. Retrieved from https://www.sciencedirect.com/science/article/pii/S0142961213000343

Brunner, E. (2013). Zur Rolle von beta 1 Integrin und von Neural cell adhesion molecule (NCAM) in der heterotypen Schwannzell-Tumorzell-Adhäsion im Pankraeskarzinom. Retrieved from https://edoc.ub.uni-muenchen.de/15563/1/Brunner_Eva_Dorothea.pdf

Choi, C., & Hao, L. (2013). Mechanism for the endocytosis of spherical nucleic acid nanoparticle conjugates. Proceedings of the  …. Retrieved from https://www.pnas.org/content/110/19/7625.short

Cui, W., Zhang, J., & Zhang, C. (2013). Control of Spontaneous Activation of Rat Oocytes by Regulating Plasma Membrane Na+/Ca2+ Exchanger Activities. Biology of  …. Retrieved from https://www.biolreprod.org/content/early/2013/05/13/biolreprod.113.108266.short

Dietmann, A., Millonig, A., & Combes, V. (2013). Effects of< i> Aggregatibacter actinomycetemcomitansleukotoxin on endothelial cells. Microbial  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S088240101300065X

Fernández, I., Gómez, P., & Parodi, J. (2013). Chilean crude extract of Ruta graveolens generates vasodilatation in rat aorta at cellular subtoxic concentrations. Retrieved from https://www.scirp.org/journal/PaperDownload.aspx?paperID=26925

Furtado, J., Ashander, L., & Mohs, K. (2013). Toxoplasma gondii Migration within and Infection of Human Retina. PloS one. Retrieved from https://dx.plos.org/10.1371/journal.pone.0054358

Hatanaka, Y., & Yamauchi, K. (2013). Excitatory cortical neurons with multipolar shape establish neuronal polarity by forming a tangentially oriented axon in the intermediate zone. Cerebral Cortex. Retrieved from https://cercor.oxfordjournals.org/content/23/1/105.short

Henkels, J., Oh, J., Xu, W., & Owen, D. (2013). Spatiotemporal Mechanical Variation Reveals Critical Role for Rho Kinase During Primitive Streak Morphogenesis. Annals of biomedical  …. Retrieved from https://link.springer.com/article/10.1007/s10439-012-0652-y

Jiao, G., Cao, X., Cui, W., Lian, H., & Miao, Y. (2013). Developmental Potential of Prepubertal Mouse Oocytes Is Compromised Due Mainly to Their Impaired Synthesis of Glutathione. PloS one. Retrieved from https://dx.plos.org/10.1371/journal.pone.0058018

Jr, E. B., Setti, A., & Vingris, L. (2013). Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques. Journal of assisted  …. Retrieved from https://link.springer.com/article/10.1007/s10815-013-9989-x

Kim, Y., Pourgholami, M., & Morris, D. (2013). Effect of shell-crosslinking of micelles on endocytosis and exocytosis: acceleration of exocytosis by crosslinking. Biomaterials  …. Retrieved from https://pubs.rsc.org/en/content/articlehtml/2013/bm/c2bm00096b

Lancaster, O., & Berre, M. Le. (2013). Mitotic Rounding Alters Cell Geometry to Ensure Efficient Bipolar Spindle Formation. Developmental cell. Retrieved from https://www.sciencedirect.com/science/article/pii/S1534580713001858

Li, X., Uchida, M., Alpar, H., & Mertens, P. (2013). Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells. Biolistic DNA Delivery. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-110-3_10

Ma, X., Wang, X., Zhou, M., & Fei, H. (2013). A Mitochondria-Targeting Gold–Peptide Nanoassembly for Enhanced Cancer-Cell Killing. Advanced healthcare materials. Retrieved from https://onlinelibrary.wiley.com/doi/10.1002/adhm.201300037/full

Mongkolchaipak, S., & Vutyavanich, T. (2013). No difference in high-magnification morphology and hyaluronic acid binding in the selection of euploid spermatozoa with intact DNA. Asian journal of andrology. Retrieved from https://www.nature.com/aja/journal/vaop/ncurrent/full/aja2012163a.html

Saade, C. (2013). Rod Photoreceptors Protect from Cone Degeneration-Induced Retinal Remodeling and Restore Visual Responses in Zebrafish. The Journal of  …. Retrieved from https://www.jneurosci.org/content/33/5/1804.short

Scarano, W., Duong, H., & Lu, H. (2013). Folate-Conjugation to Polymeric Micelles via Boronic Acid Ester to Deliver Platinum Drugs to Ovarian Cancer Cell lines.  …. Retrieved from https://pubs.acs.org/doi/abs/10.1021/bm400121q

Silberberg, Y., & Pelling, A. (2013). Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces. Cellular and Subcellular Nanotechnology. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-336-7_18

Suraniti, E., Vajrala, V., & Goudeau, B. (2013). Monitoring Metabolic Responses of Single Mitochondria within PDMS Wells: Study of their Endogenous NADH Evolution. Analytical  …. Retrieved from https://pubs.acs.org/doi/abs/10.1021/ac400494e

Thrasher, A., Stride, E., Mahadevan, L., & Charras, G. (2013). The cytoplasm of living cells behaves as a poroelastic material. Retrieved from https://www.researchgate.net/publication/234067688_The_cytoplasm_of_living_cells_behaves_as_a_poroelastic_material/file/79e4151295d3a3b280.pdf

Torres-Mapa, M. (2013). Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells. SPIE  …. Retrieved from https://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1669329

Vos, A. De, Velde, H. Van de, & Bocken, G. (2013). Does intracytoplasmic morphologically selected sperm injection improve embryo development? A randomized sibling-oocyte study. Human  …. Retrieved from https://humrep.oxfordjournals.org/content/28/3/617.short

Wang, J., Berg, K., & Høgset, A. (2013). Photophysical and photobiological properties of a sulfonated chlorin photosensitiser TPCS2a for photochemical internalisation (PCI). Photochemical &  …. Retrieved from https://pubs.rsc.org/en/content/articlehtml/2012/pp/c2pp25328c

Weinert, S. (2013). The lysosomal transfer of LDL/cholesterol from macrophages into vascular smooth muscle cells induces their phenotypic alteration. Cardiovascular …. Retrieved from https://cardiovascres.oxfordjournals.org/content/97/3/544.short

Yuseff, M., & Lennon-Dumenil, A. (2013). Studying MHC class II Presentation of Immobilized Antigen by B Lymphocytes. Antigen Processing. Retrieved from https://link.springer.com/protocol/10.1007/978-1-62703-218-6_39

RelatedItems

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

Keep in Touch

We promise NEVER to share your details with anyone. You can opt out at any time.

Downloads

Main CatalogueSurgical ToolsAnimal Physiology
MDS

Browse Online...
Hardcopy

Browse Online...Browse Online...