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SI-BF-100

SI-BF-100

Biofluorometer

  • Overview
  • Specifications
  • Accessories
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Overview

SI-BF100 Probe

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SI-BF-100 DataSheet
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SI-BF-100 Instruction Manual
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SI-BF-100 Application Note
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Belz et al, SPIE 9702, 2016
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The new SI-BF100 is an LED-based fluorometer for life science applications. It is ideally suited for ratiometric calcium detection (FURA-2) and ATPase detection (via NADH fluorescence). With up to seven LED modules (wavelengths), the SI-BF-100 covers many fluorometric applications in neuroscience and cell biology.

  • LED light sources require less power, give off less heat and are more compact and affordable.
  • Sampling rates up to 1kHz (1,000 ratios/second maximum). At lower speeds, signal averaging is used for noise reduction.
  • Two auto ranging photomultiplier inputs allow you to monitor multiple wavelengths from a single emission output that can be comprised of any wavelength of light for which an LED module is available.
  • Using a separate reference channel, ultra-stable, continuous ratio calculations automatically compensate for LED intensity drift. This ensures less noise and produces more accurate measurements.
  • Application-specific probes are available for existing tissue baths and cuvette systems.
  • Ratio noise is
  • The warm up time of less than one minute is a dramatic improvement over the common 20–60 minutes required by xenon or mercury light sources
  • Replace the emission filter easily or change the LED modules to transform the SI-BF100 into a general purpose fluorometer for many other applications
  • Unique fiber optic coupling probe allows for highly efficient transfer of light and ease of placement. Custom probes fit your existing systems.


How it works

Up until now, calcium imaging systems have been required to compensate for errors and noise introduced by the complexity of their design. The systems require mechanical filters and use expensive xenon or mercury light sources. The beauty of the SI-BF100 is its simplicity. The elegance of its design reduces the noise introduced into the system and the errors inherent in traditional designs.

Monochromatic LED light sources using WPI patented technology eliminate the need for complex and expensive white light sources and filter wheels. Because the LED modules can be pulsed, sampling frequencies up to 1,000 cycles per second are possible. The LED light source emits specific excitation frequencies which travel through the probe. The excitation light can be comprised of any wavelength of light for which an LED module is available. The probe returns a single emission output to one or two photomultiplier inputs on the front of the SI-BF-100, which are independently filtered for specific wavelengths. This design allows you to monitor multiple wavelengths from a single emission output. The LED light source in the WPI design makes this ratiometric fluorometer more compact, energy efficient and affordable. As added benefits, the low-power light source produces much less heat, and it warms up in less than one minute!

This incredible design is not limited to calcium imaging either. By simply replacing the 510nm emission filters in front of the photomultipliers with the desired wavelength filters, your SI-BF100 becomes a general purpose fluorometer for any application you can imagine. Changing a filter involves removing the two screws that hold the filter carriage on the face of the SI-BF-100, swapping the filter and reinstalling the integral SMA/filter carriage.

Specifications

Ca2+Excitation Wavelength 365, 415 nm for Fura-8 (others available)
Ca2+Emission Wavelength 525nm (others available)
ATPase (NADH) Excitation Wavelength 360nm (others available)
ATPase (NADH) Emission Wavelength 470nm, 570nm (others available)
Fiber Optic Light Input/Output LLG or SMA terminated
Sampling rate Up to 1000 ratios/second
Ratio Noise < 0.5 peak-to-peak
Signal Drift < 0.1 unit/hours
Analog Output Range 0–10V (continuous, equivalent to a ratio 0–10)
Analog Output Impedance 100Ω
Power 12VDC, 0.5A, (universal power supply, 110/240VAC)
Warm Up Time  
Dimensions 3.5"H x 17"W x 13"D (88 x 431 x 330mm)

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Citations

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