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Researcher, ICBAS - Universidade do Porto

WPI provided a very professional, supportive and quick service upon purchasing several pieces of scientific equipment.
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MIRUS-EVO-PRO

MIRUS-EVO-PRO

The Mirus™ Evo Nanopump is a microfluidic syringe pump PC controlled via VenaFluxAssay Software.


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  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

MIRUS EVO PUMP

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Quick Start - MIRUS Washout
/ Download as PDF

Quick Start - MIRUS Connect Tubing
/ Download as PDF

Quick Start - VenaFlux Assay
/ Download as PDF

Features:

  • Includes MultiFlow8 for precision flow splitting with equal flow rate in each channel.
  • MultiFlow8 contains 8 valves which can be switched on/offi independently.
  • Higher throughput enabling 8 assays in parallel.
  • Patented flow damper to decrease syringe pump pulses - particularly important when connecting to microfluidic biochips to prevent pusling/jerking motion comapred to standard syringe pump.
  • Flow rate: 100nL/min - 10mL/min ± 1%.
  • Standard syringes: 50µL - 5mL.
  • Dead volume: ~600µL.
  • Flow direction reversible.
  • PC controlled via VenaFluxAssay software.
  • Mirus Evo may be sold separately without MultiFlow8. MultiFlow8 can be purchased later if required.
  • Includes tubing kit for Vena8 biochips or alternative tubing kits for connection to any microfluidic biochip.

Applications: Microfluidic applications, single cell analysis, Microfluidic syringe pump for cell analysis under shear flow in biochips. Suitable for cell samples and whole blood samples.

Specifications

MultiFlow8TM

Capable of executing up to 8 assays in parallel in Vena8TM biochips resulting in an 8-channel syringe pump.

Shear Stress Range for cell suspension

0.05 - 10 dyne/cm2steps of 0.05 dyne/cm2 (100 mL syringe)

Shear Stress Range for whole blood*

2.25 - 450 dyne/cm2 (1 mL syringe)

Volumetric Flow Rates

100 nL/minute - 20 μL/minute (100 mL syringe)

Dead Volume

600 μL

Sample Volume Increments

  • Freely adjustable
  • Pulsatile flow option available

Valve Switching Time

30 ms max (at 20°C, 2Hz, with air under 10psi pressure)

Working Pressure

30 psi – 2 bars maximum

Linear Velocity Range**

10 μm/s to 10 cm/s

Flow Direction

Reversible

Sample Volume Aspiration Accuracy

±1%

Shear Stress Accuracy

±0.5%

Sample Volume Aspiration Precision

 

Shear Stress Precision

 

External Trigger

2 inputs and 2 outputs external trigger for better operation with external units and softwares

Software Control

Integrated VenaFlux Assay software

Dimensions

  • Pump:  84mm (W) x 180mm (D) x 192.5mm (H)
  • MultiFlow8:  140mm (W) x 35mm (D) x 140mm (H)
 Weight
  • Pump:  ~2kg
  • MultiFlow8: 

Accessories

Vena8 Endothelial+

Vena8 Endothelial+

The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on c...

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Vena8 Fluoro+

Vena8 Fluoro+

The Vena8 Fluoro+™ biochip is ideal for rolling and adhesion assays on protein coatings...

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VenaT4

VenaT4

The VenaT4™ biochip for chemotaxis, transmigration and invasion assays. Compatible with...

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Vena8 with Glass Coverslip (Adhered / Not Adhered; Treated / Not Treated)

Vena8 with Glass Coverslip (Adhered / Not Adhered; Treated / Not Treated)

The Vena8™ biochip with/without glass coverslip adhered; with/without treatment for cel...

View details...

Citations

Aelst, B. Van, & Devloo, R. (2015). Ultraviolet C light pathogen inactivation treatment of platelet concentrates preserves integrin activation but affects thrombus formation kinetics on collagen in vitro. …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1111/trf.13137/full

Calmer, S., Ferkau, A., Larmann, J., & Johanning, K. (2014). Desmopressin (DDAVP) improves recruitment of activated platelets to collagen but simultaneously increases platelet endothelial interactions in vitro. Platelets. Retrieved from https://www.tandfonline.com/doi/abs/10.3109/09537104.2013.767442#

Curcic, S., Holzer, M., Frei, R., & Pasterk, L. (2015). Neutrophil effector responses are suppressed by secretory phospholipase A 2 modified HDL. … et Biophysica Acta (BBA …. Retrieved from https://www.sciencedirect.com/science/article/pii/S1388198114002492
Dominical, V., Vital, D., & O’Dowd, F. (2015). In vitro microfluidic model for the study of vaso-occlusive processes. Experimental …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0301472X14007504

Kashanin, D., Shvets, I., Williams, V., & O’dowd, F. (2014). Method for measuring the migration of cells in a channel under the influence of an analyte. US Patent 8,802,391. Retrieved from https://www.google.com/patents/US8802391

Konya, V., Peinhaupt, M., & Heinemann, A. (2014). Adhesion of Eosinophils to Endothelial Cells or Substrates Under Flow Conditions. Eosinophils. Retrieved from https://link.springer.com/protocol/10.1007/978-1-4939-1016-8_13

Natoni, A., Moschetta, M., Glavey, S., & Wu, P. (2014). Multiple Myeloma Cells Express Functional E-Selectin Ligands Which Can be Inhibited Both in-Vitro and in-Vivo Leading to Prolongation of Survival in a. Blood. Retrieved from https://www.bloodjournal.org/content/124/21/4718.abstract

Salles-Crawley, I., Monkman, J., & Ahnström, J. (2014). Vessel wall BAMBI contributes to hemostasis and thrombus stability. Blood. Retrieved from https://www.bloodjournal.org/content/123/18/2873.short

Shaker, M., Colella, L., Caselli, F., Bisegna, P., & Renaud, P. (2014). An impedance-based flow microcytometer for single cell morphology discrimination. Lab on a Chip. Retrieved from https://pubs.rsc.org/en/content/articlehtml/2014/lc/c4lc00221k

Tischer, A., & Madde, P. (2014). A molten globule intermediate of the von Willebrand factor A1 domain firmly tethers platelets under shear flow. Proteins: Structure, …. Retrieved from https://onlinelibrary.wiley.com/doi/10.1002/prot.24464/full

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GlaxoSmithKline
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Imperial College
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University of Oxford

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