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Filaments

Filaments

Box Filaments and Through Filaments


Appropriate filament selection depends on your research application, but a general guideline for filaments is as follows:

Box Filaments - Recommended for small to large diameter borosilicate glass, double barreled, or aluminosilicate glass. Box filaments are particularly suitable for patch pipettes, slice preparations, and for sharp electrodes and microinjection pipettes where gradual parallel walls would aid cell penetration. When using a box filament, the size of the square box should be approximately 1.0mm to 1.5mm larger than the outside diameter of the glass that you will be using.

For IVF and ICSI applications, a 2.5mm x 4.5mm box filament (FB245B) is recommended. For pronuclear injection work, we recommend a 2.5 mm x 2.5 mm box filament (FB255B). Call for more specific advice or to request a combination of settings, filaments, and glass for your application.

Trough Filaments - Excellent general purpose filaments and are recommended for creating longer slice patch pipettes and sharp electrodes. The trough filament is not ideal for making patch pipettes or when using glass over 1.2 mm OD. If very short tapers are required for patch electrodes or for microinjection, a 2.5 mm or 3 mm box filament is recommended.

Custom Filaments - A custom filament can be created if your pipette needs can not be met by one of the standard box or trough filaments. Please call Sutter for details.

Sutter pre-programs the P-97 and P-1000 with a 2.5 mm x 2.5 mm box filament unless an alternative filament is requested.

For either shape of filament (box or trough), increasing the filament width tends to increase the length of the pipette taper. If there are specific questions as to the sizes needed, please call and our technical support staff will guide you in your choice.

 



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FB215B

FB215B

2mm Box Filament,1.5mm Wide


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FB220B

FB220B

2mm Box Filament, 2mm Wide


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FB230B

FB230B

2mm Box Filament, 3mm Wide


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FB245B

FB245B

2.5mm Box Filament, 4.5mm Wide


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FB255B

FB255B

2.5mm Box Filament, 2.5mm Wide


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FB315B

FB315B

3mm Box Filament, 1.5mm Wide


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FB320B

FB320B

3mm Box Filament, 2mm Wide


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FB330B

FB330B

3mm Box Filament, 3mm Wide


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FT315B

FT315B

Trough Filament, 1.5mm Wide


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FT320B

FT320B

Trough Filament, 2mm Wide


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FT330B

FT330B

Trough Filament, 3mm Wide


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FT345B

FT345B

Trough Filament, 4.5mm Wide


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13834

13834

2.5 mm Box Filament, 2.5 mm wide


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14074

14074

3 mm Box Filament, 2 mm wide


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PF30N

PF30N

Nichrome filament (3 turns)

For P-30 pullers BEFORE SN# P30-680


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PF30N-4

PF30N-4

Nichrome filament (4 turns)

For P-30 pullers AFTER SN# P30-679

With the P30N-4 you can 1) use lower heat settings, 2) pull longer tapers (2-3cm) and 3) use aluminosilicate glass. If you need to pull very short tapers, the P30N is recommended.


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PF30T15

PF30T15

1.5mm wide through filament for P-30 puller


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PF30T20

PF30T20

2.0mm wide through filament for P-30 puller


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PF30T30

PF30T30

3.0mm wide through filament for P-30 puller

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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