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PV850

PV850

Microinjector with External Pressure Source (pressure source not included)



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Overview

PV850 PV850 interface

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Instruction Manual
/ Download as PDF

Compensation pressure accuracy
/ Download as PDF

Features & Benefits

  • High precision for reproducible injections – microliters to picoliter
  • Touch screen display and control knob with intuitive user-interface
  • Injection Counter
  • Injection can be triggered manually using the touchscreen, by foot switch or by a computer-controlled TTL pulse
  • Small footprint takes up very little bench space

The PV850 is designed to inject very small quantities of fluids, such as drugs, into cells or small organelles.Two different positive pressures may be applied – one for ejection at high pressure and a second, lower pressure to prevent back filling of the pipette by capillary action.

Applications

  • Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
  • Pre- and post-implantation in embryos of various species – mice, rats, monkeys, bovine, pigs, zebrafish, etc.

Overview

The PV850 requires an external pressure supply (not included) and is the ideal choice for applications that require serial experiments with a large number of injections.

Designed to simplify intracellular injection and a variety of other microinjection tasks, the PV850 uses carefully regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive “compensation” pressure to the injecting pipette between injection pulses to prevent fluid uptake through capillary action.

Timing, injection pressure and compensation pressure are adjusted independently using the touch screen interface and control knob. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting. The injection pressure interval is triggered by using a foot switch, manually, or a computer-controlled TTL pulse.

The PV850 offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically.

The display provides easy visibility of key data settings including Injector Pressure, Compensation Pressure, Injection Time, Pump Injection Mode and Injection Count

WPI's New PV850 Microinjector: Setting Up the Hardware

 

WPI's New PV850 Microinjector: Using the Software

PV850 Microinjector Setup Tip

Seting Up The PV850 System

Setting up the PV850 microinjection system

 

Comparison Of Microinjectors

Comparison of Microinjectors

Specifications

PRESSURE

Pressure Input 0-150 PSI
Injection Pressure Range, Controlled 0.07-87 PSI
Injection Pressure Increment 0.1 PSI
Regulator Accuracy ±0.1 PSI
Input Connector Quick Connect (1/4" OD tubing)
Output Connector Quick Disconnect (compatible with capillary kit 300753)
Pulse Width

Time Interval: 0.001 to 2.000 s

Time Increment: 0.001 s

COMPENSATION (HOLD/BACKFILLING) PRESSURE

Compensation Pressure Range 0.07-87 PSI
Compensation Pressure Increment 0.1 PSI

 

CONNECTIONS INCLUDED

Input Kit 10' nylon tubing (0.25" OD, 1000 PSI), one 1/4" female NPT adapter
Output Kit Capillary kit 300753

 

PHYSICAL SPECS

Power 90-264 VAC, 50/60Hz
Dimensions 213 W x 207 D x 250 H mm (8.4 x 8.1 x 9.8")
Shipping Weight 5 kg

 

Accessories

Citations

RelatedItems

Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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