How to Run & Stop a Scheduled Sequence with EVOM™ Auto GxP

EVOM™ Auto with the GxP module lets you perform automated transepithelial electrical (TEER) resistance with confidence that your data security complies with the FDA 21 CFR Part 11 regulations. Here we will see how to run or stop a scheduled measurement sequence.

The data acquisition functions of the EVOM™ Auto GxP may be repeated at a user-defined time interval when multiple scans of a plate are needed. The scheduled operations are displayed on the right side of the Experiment window in the Current State area. The control buttons for scheduling are in the Schedule Controls area on the right side of the window.

1. Select Experiment from the main menu to access the Experiment window
2. In the Schedule Controls area, press the Set Period button to set the length of time in between well plate scans. Enter a four-digit number (HHMM) to set the hours and minutes or click on the clock icon to reveal a graphical helper. Use the spinner to select the hours from 1 to 23. The first 12 hours are on the outside of the spinner, and the inner spinner is used to set 13 – 23 hours. After you make that selection, the spinner displays minutes. Use the dial to set the number of minutes in the period. Then, press the SAVE button
3. Press the Set Cycles button to set the number of scans to be performed according to the current sequence. Use the + and – buttons to increment or decrement the number of scans. Then, press OK
4. Press the Run button in the Schedule Controls area to start the first scan. Enter your comment and your EVOM™ Auto GxP Client password. If you don’t want to add a comment, you may select the No Comment checkbox. If you do, default text appears in the comment line. Press the Run button. After that scan, the Time and Cycle counters become active in the Current State area on the right side of the window. They will continue counting down until they reach zero. Then, measurements stop. When a scan is finished, the data is saved, and the plate number (run number) increments
5. To stop the scheduled sequence, press the Stop button in the Schedule Controls area. Enter your comment and your EVOM™ Auto GxP Client password. Then, press the Stop button. The current scan will continue until it completes the cycle. The Stop and Pause buttons become active in the Plate Controls area
6. If you also need to stop the scan, you must press the Plate Controls Stop button. Enter your comment and your EVOM™ Auto GxP Client password. Then, press the Stop button. Measurements continue until the scan reaches the end of the active column. The data is automatically saved, and the saved data includes all the collected data, including the last active row scanned