How to Run, Pause & Stop an EVOM™ Auto GXP Experiment

WPI’s EVOM™ line of transepithelial electrical resistance (TEER) measurement systems is the industry standard with thousands of citations in peer-reviewed journals spanning over 40 years. Our newest automated TEER measurement unit, the EVOM™ Auto, works with both 24- and 96-well plates, and now it is available with an optional GxP module to ensure 21 CFR Part 11 compliance to satisfy FDA requirements. In this video you can see how to run, pause, and stop an experiment.

After you have defined an EVOM™ Auto sequence, you can begin running an experiment.

To begin running an experiment:

1. Select Experiment from the main menu. The Experiment window appears
2. Select the Plate Name drop-down in the Details area on the right side of the screen. Choose from the list of existing
3. Select the Sequence Name drop-down, the second drop-down in the Details area on the right side of the screen. A list of existing sequences appears. Select the appropriate sequence
4. You may add comments for the data run being collected. Click inside the Notes text block in the Details section. All your comments must be added before you click the Run button
5. Use the blank data table with no data values to select which wells you want to measure
6. Press the Run button. Enter your comment, and your EVOM™ Auto GxP Client password. If you don’t want to add a comment, you may select the No Comment checkbox. If you do, default text appears in the comment line. Then, press the Run. The sequence is executed. A brief message in the lower left corner indicates that the experiment has started. If you defined the blank container, the data displayed represents the difference between the baseline and the actual value measured
7. You may pause or stop the experiment, if necessary. If you pause or stop the experiment, you will be prompted for a comment and your password
8. If you stop an experiment, the autosampler finishes the data collection on the present column and lifts the electrode array out of the well plate. The data acquisition is halted. The sequence may be restarted.
9. If you pause the experiment, it finishes the data collection on the active column and lifts the electrode array out of the well plate. Continue the data acquisition run by pressing the Resume button. You will again be prompted to enter a comment and your password