EVM-EL-03-01-02

Overview

For TEER measurement of epithelial and endothelial cell cultures in individual cups

• Compatible with legacy meters including EVOM3 and EVOM Manual™ meters (see options section for compatible with legacy models including the EVOM2 and Millipore Millicell ERS2 meters)

Benefits & Features

• Stability and reproducibility superior to the STX4 electrodes to 1% tolerance
• Symmetrical electrode pattern disperses test current uniformly
• Tri-leg supports offer mechanical stability and the membrane is held parallel to the electrodes
• Simple test procedure to verify electrode performance
• Adjustable apical electrode height
• Crystal clear glass chamber allows visualization of apical electrode positioning
• The new EndOhm chamber upper mount is made of polycarbonate and unaffected by alcohol
• The glass chamber is easier to clean and more scratch resistant than the prior version. The EndOhm is not recommended for incubator use due to risk of the glass cracking
• Crystal clear glass chamber allows visualization of apical electrode positioning
• Options for 6, 12 or 24 well plates with removable inserts

Applications

• TEER measurement for removable culture cup systems using EVOM meters for endothelial and epithelial cell cultures

Options

For Legacy Meters Including EVOM, EVOM2™ and Millipore ERS and ERS2 meters

Part #	Description
ENDOHM-24G-SNAP	EndOhm for 24mm and Costar Snapwell Culture Cups (6 wells per plate)
ENDOHM-12G	EndOhm for 12mm Culture Cups (12 wells per plate)
ENDOHM-6G	EndOhm for 6mm Culture Cups (24 wells per plate)

 

For EVOM3 and EVOM Manual™ meters

Part #	Description
EVM-EL-03-01-03	EndOhm for 24mm and Costar Snapwell Culture Cups (6 wells per plate)
EVM-EL-03-01-02	EndOhm for 12mm Culture Cups (12 wells per plate)
EVM-EL-03-01-01	EndOhm for 6mm Culture Cups (24 wells per plate)

 

TEER measurement in individual cups

Using WPI’s EVOM resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers in culture cups. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture.

Make more precise measurements with Endohms

Endohm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with STX4 electrodes. The background resistance of a blank insert is reduced from 150 Ω (when using WPI’s hand-held STX4 electrodes) to less than 5 Ω. With Endohm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 Ω with STX4 electrodes (depending on the experience of the user) to 1-2 Ω. Compared with other resistance measurement methods, Endohm with EVOM meters offer a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM Meter, errors owing to electrode polarization or membrane capacitance are largely eliminated. Endohm together with EVOM Manual™ offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Corning, Millipore, Nunc, Greiner and BD Falcon have been tested. Endohm chambers may be sterilized with EtO, alcohol or a bactericide; not autoclavable.

NOTE: EndOhm chambers have Ag/AgCl electrodes. If you are measuring for extended periods of time, you should consider any potential cytotoxic issue from long term exposure to silver to your cells.

Resources

Knowledge base - EVOM™ Electrodes & EndOhm Chambers Articles

Videos

 

Specifications

Compatibility Charts

The ENDOHM-12 (#EVM-EL-03-01-02) is compatible with the following chambers:

Corning	Millipore	Membrane Diameter (mm)	Growth Surface Area (cm²)	Membrane Pore Size (μm)
3401		12	1.12	0.4
3402	PITP01250	12	1.12	3.0
3403	PITT01250	12	1.12	3.0
3493		12	1.12	0.4
3494		12	1.12	3.0
3460	PIHT15R48* PET Insert	12	1.12	0.4
	PIRP15R48* PET Insert	12	1.12	1.0
3462	PISP15R48* PET Insert	12	1.12	3.0
	PIMP15R48* PET Insert	12	1.12	5.0
	PIEP30R48* PIEP15R48* PET Insert	12	1.12	8.0

* The tri-supports legs must be balance correctly so that the filter is parallel to the electrodes.

Nunc	Pore size (μm)	Culture area (cm²)
140652	0.4	1.13
140654	3.0	1.13
140656	8.0	1.13

 

ThinCertTM	Membrane material	Pore size [µm]	Pore density [cm-2]	Optical membrane properties	TC surface treatment/Sterile	Multiwell plates/ThinCertTM per box
665640	PET	0.4	1 x 108	translucent	+/+	4/48
665641	PET	0.4	2 x 106	transparent	+/+	4/48
665610	PET	1.0	2 x 106	transparent	+/+	4/48
665630	PET	3.0	0.6  x 106	transparent	+/+	4/48
665631	PET	3.0	2 x 106	translucent	+/+	4/48
665638	PET	8.0	0.15 x 106	translucent	+/+	4/48

 

Millicell	Pore size (μm)	Qty/pk
MCHT12H48	0.4	48
MCRP12H48	1.0	48
MCSP12H48	3.0	48
MCMP12H48	5.0	48
MCEP12H48	8.0	48

 

BD Falcon	Membrane material	Pore size [µm]	Pore density [cm-2]	Optical membrane properties	TC plate (#wells)
353180	PET	0.4	2.0 ± 0.2 x 106	transparent	12
353103	PET	1.0	1.6 ± 0.6 x 106	transparent	12
353181	PET	3.0	8 ± 2 x 105	transparent	12
353182	PET	8.0	6 ± 2 x 104	translucent	12
353494	PET	0.4HD	100 ± 10 x 106	translucent	12
353292	PET	3.0HD	2.0 ± 0.2 x 105	translucent	12

Accessories

Citations

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