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REMS

REMS

Automated Tissue Resistance Measuring System

  • Overview
  • Specifications
  • Accessories
  • Citations
  • Related Products

Overview

REMS

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REMS Application Note
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Instruction Manual
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Data sheet
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PC-controlled high throughput TEER measurement for epithelial monolayer

Automates TEER measurement and data logging for use with HTS well plates

  • PC controlled positioning
  • Data acquisition in LabView
  • Manufacturer specific electrodes available for 24 and 96-well HTS plates
  • Plate configuration files and sample sequences are user definable
  • Two user-defined rinse locations
  • Manual mode
Benefits
  • Speed—capable of acquiring TEER data on a 96 well plate in less than five minutes
  • Automation reduces the possibility for human error

Applications

  • Automated measurement and data logging of TEER for 24 and 96 well HTS culture plates

The REMS AutoSampler automates measurements of TEER epithelial or endothelial monolayers cultured on HTS well plates. It is a PC-controlled tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation. Automated measurement of tissue resistance in cell culture microplates provides the advantages of speed, precision, decreased opportunity for contamination and the rapid availability of measured resistance data.

The main components of the REMS AutoSampler include: the robotic sampler that moves the electrode over each well of the microplate, the electrode which is located on the robotic arm, a base plate for the 24 and 96 well tray, a Windows-based data acquisition card, the REMS electrode interface unit and the REMS software to operate the system on a Windows-based computer.

Automate TEER measurements

The REMS AutoSampler automates TEER?measurements that would otherwise be performed manually with WPI’s EVOM2™ Epithelial Voltohmmeter. Automated tissue resistance measurements up to 20 kΩ can be performed on 24 or 96 well HTS microplates. See WPI’s website for manufacturer plate compatibility.

Precisely and reproducibly positions electrode

The REMS AutoSampler will automatically measure and record tissue resistance from a user-specified matrix of culture wells on the microplate. According to the specified sequence, the robotic arm moves over the identified wells taking TEER measurements. By means of an x-y-z locating system, the electrode is positioned precisely into the well. The ability of the REMS AutoSampler to reproducibly position the electrode contributes to consistent TEER measurements. TEER data is incrementally stored as the electrode moves from one well to the next.

Compact electrode pair

The use of AC current to measure resistance provides several advantages over DC current, including:

  • Absence of offset voltages on measurements
  • There is a zero net current being passed through the membrane and, therefore, it is not adversely affected by a current charge
  • No electrochemical deposition of electrode metal.

Rinse and calibration check stations

The REMS AutoSampler also features two rinse stations. If occasional rinsing of the REMS electrode is required it may be sent to a rinse station by pressing the rinse station button on the menu bar.

Compatible Plates

The following plates can be used with the REMS system:

REMS-24 REMS-24M REMS-96 REMS-96 REMS-96C 
Corning HTS Transwell BD Falcon HTS MultiWell Millicell-24 Millipore HTS96 Multiwell BD Falcon Corning HTS Transwell
3378
3379
3396
3397
3398
3399
351180
351181
351182
351183
351184
351185
354803
354804

PSHT010R1
PSST010R1
PSMT010R1
PSET010R1
PSRP010R1
PSRP010R5
PSHT010R5

 

PSHT004R1
PSRP004R1
PSRP004R5
PSHT004S5
PSHT004R5

351130
351131
351161
351162
351163
351164
353938
3380
3392
3381
3391
3385
3386
3387
3388
3374
3384

 

Sample Plates

Corning #3378 24-well plate
Click to enlarge.
BD Falcon 96-well plate
Click to enlarge.
Millipore Multiscreen CaCo2
Click to enlarge.
Corning 3391 HTS Traswell 96 plate
Click to enlarge.
millicell-24 well plate
Click to enlarge.

This is a Corning #3378 24-well plate that may be used with the REMS-24 or the STX100C.

The BD Falcon 24-well plate is virtually identical to this one. For use with REMS-24 or STX100F.

 This is a BD Falcon 96-well plate that may be used with the REMS-96.

 This is a Millipore Multiscreen CaCo2 that may be used with the REMS-96 or the STX100M.  This is a Corning 3391 HTS Traswell 96 plate that may be used with the REMS-96C or the STX100C96. This is a Millicell-24 Cell Culture Insert Plate that may be used with the REMS-24M

 

 

 



The video below demonstrates the REMS robotic automated TEER measurement system and discusses the recent updates made to the software.

 

Specifications

MEMBRANE RESISTANCE RANGE 0 to 2000Ω and 0 to 20kΩ
AC SQUARE WAVE CURRENT +/- 20μA @ 12.5 Hz
ELECTRODE POSITIONING Resolution in X, Y and Z: +/- 1mm
ELECTRODE PERFORMANCE Repeatability in X, Y and Z: +/- 0.25mm
ELECTRODE ARM SPEED X- and Y-axis: 250mm/sec Z-axis: 247.3mm/sec
TYPICAL MEASUREMENT TIME 24-WELL 1 min, 10 sec
SCAN PATTERN Choice of any well pattern sampling
 LINE VOLTAGE User specified: 100/120 V or 220/240 V
DIMENSIONS 53.5 x 43.7 x 37.1 cm (21.09 x 17.19 x 14.63 in.)
WEIGHT 24 kg (52 lb)

The system includes robot sampler, base plate, data acquisition board, computer, monitor, keyboard, mouse, software for Windows XP or Vista, and electrode for either 24-well plate (Corning Costar HTS Transwell-24 or Falcon HTS Multiwell) or 96-well plate (Millipore Multiscreen CaCo). Please specify which plate when ordering.

Accessories


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REMS-96C

REMS-96C

Replacement REMS STX Electrode for Corning 96-well Plate

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REMS-24

REMS-24

Replacement REMS STX Electrode for 24-well HTS Plate. Use with REMS system.

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REMS-96

REMS-96

Replacement REMS STX Electrode for Millipore 96-well Plate. Use with REMS system.

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Citations

Duff, T., Carter, S., & Feldman, G. (2002). Transepithelial resistance and inulin permeability as endpoints in in vitro nephrotoxicity testing. ATLA- …. Retrieved from https://ecvam-dbalm.jrc.it/publication/Duff.pdf

Levtchenko, E. (2013). NOVEL CONDITIONALLY IMMORTALIZED HUMAN PROXIMAL TUBULE CELL LINE EXPRESSING FUNCTIONAL INFLUX AND EFFLUX TRANSPORTERS. US Patent  …. Retrieved from https://www.freepatentsonline.com/y2013/0130223.html

Tambuwala, M., Cummins, E., & Lenihan, C. (2010). Loss of prolyl hydroxylase-1 protects against colitis through reduced epithelial cell apoptosis and increased barrier function. Gastroenterology. Retrieved from https://www.sciencedirect.com/science/article/pii/S0016508510010115

Walk, J., Eichinger, T., & Balbach, S. (2011). Online TEER measurement in a system for permeation determination by means of a flow-through permeation cell (FTPC) having structurally integrated electrodes. EP Patent  …. Retrieved from https://www.freepatentsonline.com/EP2302353.html

Wempe, M., Wright, C., & Little, J. (2009). Inhibiting efflux with novel non-ionic surfactants: Rational design based on vitamin E TPGS. International journal of  …. Retrieved from https://www.sciencedirect.com/science/article/pii/S0378517308007886

RelatedItems

EVOM2

EVOM2

Trans Epithelial electric resistance (TEER) Measurements

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Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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