STX HTS EVOM™ Electrode for TEER
For high throughput screening (HTS) in cell culture filter plates
STX HTS EVOM & STX100 Instruction Manual
For high throughput screening (HTS) in cell culture filter plates
- Designed for use with 24-well HTS plate (Corning Costar and Corning Falcon) and with 96-well plates (Corning, Millipore and MatTek)
- Improved accuracy down to 5 Ω
- Sterilized with EtO, alcohol or bactericide
Options
The STX HTS EVOM electrode are available with a Mini XLR connector compatible for the EVOM Manual and EVOM3 meter. To connect with the EVOM, EVOM2™, and Millicell® ERS and ERS2 use the Mini XLR to RJ-11 connector EVM-AC-02-01-01. Use the tables below to select the required option.
STX HTS compatible with EVOM™ Manual and EVOM3
| Order code | Description |
| EVM-EL-03-03-05 | STX HTS EVOM™ Electrode for TEER in Corning 96 |
| EVM-EL-03-03-02 | STX HTS EVOM™ Electrode for TEER in Millipore 96 |
| EVM-EL-03-03-03 | STX HTS EVOM™ Electrode for TEER in Corning Biocoat/Falcon 24 |
| EVM-EL-03-03-04 | STX HTS EVOM™ Electrode for TEER in Corning Costar 24 |
| EVM-EL-03-02-05 | STX HTS EVOM™ Electrode for TEER in MatTek 96 |
STX HTS compatible with EVOM, EVOM2™, and Millicell® ERS and ERS2
Order STX HTS EVOM™ Electrode for TEER from above and add Mini XLR to RJ-11 connector EVM-AC-02-01-01.
| Order codes | Replaces Historical Electrode |
| EVM-EL-03-03-05 & EVM-AC-02-01-01 | STX100C96 - Electrode forEVOM2, Use with Corning HTS 96-Well Plate |
| EVM-EL-03-03-02 & EVM-AC-02-01-01 | STX100M - Electrode for EVOM2, Use with Millipore Multiscreen HTS 96-Well Plate |
| EVM-EL-03-03-03 & EVM-AC-02-01-01 | STX100F - Electrode for EVOM2, Use with Corning Falcon HTS Multi Well Insert System |
| EVM-EL-03-03-04 & EVM-AC-02-01-01 | STX100C - Electrode for EVOM2, Use with Corning Costar HTS Transwell-24 |
Benefits
- Smaller tip size than the STX4 electrode constructed for durability fits neatly into the keyhole shaped filter well
- Electrode design reduces chance of contamination
Applications
- STX HTS EVOM Electrodes are designed for TEER measurement in HTS culture plates using the EVOM Manual meters
Semi-permeable HTS (high throughput screening) culture plates have become a standard tool for pharmaceutical and institutional research in epithelial transport. HTS culture plates have well cups which are bonded together into a single assembly and are not removable. This makes the plates ideal for automated applications, but imposes a significant inconvenience when TEER must be measured in the absence of an automated system. WPI’s STX HTS EVOM series electrodes provide a cost-effective alternative to automation, allowing HTS well plates to be measured manually using a hand–held electrode.
The spatial orientation of an electrode during TEER measurement can have a significant effect on the resistance reading. When compared to the “chopsticks” electrode, the STX HTS EVOM series electrodes offer a technical advantage. The design of the STX HTS EVOM series electrodes guarantees spatial repeatability. Each STX HTS EVOM electrode is designed to self-align based on the form factor of the apical and basal access ports of the HTS plate. Each manufacturer of HTS culture systems has a unique form factor, and the correct STX HTS EVOM electrode to use is manufacturer specific.
WPI developed STX HTS EVOM electrodes for 24 and 96 well plates specific to Corning, Corning Falcon, Millipore and MatTek. Refer to the charts for compatibility information or contact your WPI sales representative for assistance.
Compatibility charts
The STX HTS EVOM is compatible with the following Millpore plates.
|
Corning 96 (EVM-EL-03-03-05) |
Part # | Description | Pore Size | Membrane |
 |
3380 | HTS Transwell-96 System | 1.0μm | PET |
| 3392 | HTS Transwell-96 System | 1.0μm | PET | |
| 7369 | HTS Transwell-96 System | 0.4μm | PET | |
| 3381 | HTS Transwell-96 System | 0.4μm | PC | |
| 3391 | HTS Transwell-96 System | 0.4μm | PC | |
| 3385 | HTS Transwell-96 Well Plate | 3.0μm | PC | |
| 3386 | HTS Transwell-96 Well Plate | 3.0μm | PC | |
| 3387 | HTS Transwell-96 Well Plate | 5.0μm | PC | |
| 3388 | HTS Transwell-96 Well Plate | 5.0μm | PC | |
| 3374 | HTS Transwell-96 Well Plate | 8.0μm | PET | |
| 3384 | HTS Transwell-96 Well Plate | 8.0μm | PET | |
|
Millipore 96 (EVM-EL-03-03-02) |
Part # | Description | Pore size | Membrane |
 |
PSHT004R1 | 96-well plate | 0.4um | PCF |
| PSRP004R1 | 96-well plate | 1.0um | PET | |
| PSHT004R5 | 96-well plate | 0.4um | PCF | |
| PSRP004R5 | 96-well plate | 1.0um | PET | |
| PSHT004S5 | 96-well plate | 0.4um | PCF | |
|
Corning Biocoat/Falcon 24 (EVM-EL-03-03-03) |
Part # | Description | Pore Size | Membrane |
|
|
351181 | Corning Falcon HTS Multiwell Insert System | 1.0μm | PET |
| 351183 | Corning Falcon HTS Multiwell Insert System | 3.0μm | PET | |
| 351185 | Corning Falcon HTS Multiwell Insert System | 8.0μm | PET | |
| 354803 | Corning BioCoat HTS Fibrillar Collagen Multiwell Insert System | 1.0μm | PET | |
| 354804 | Corning BioCoat HTS Fibrillar Collagen Multiwell Insert System | 1.0μm | PC | |
|
Corning Costar 24 (EVM-EL-03-03-04) |
Part # | Description | Pore Size | Membrane |
|
|
3378 | HTS Transwell-24 | 0.4μm | PET |
| 3379 | HTS Transwell-24 | 0.4μm | PET | |
| 3396 | HTS Transwell-24 | 0.4μm | ||
| 3397 | HTS Transwell-24 | 0.4μm | ||
| 3398 | HTS Transwell-24 | 3.0μm | ||
| 3399 | HTS Transwell-24 | 3.0μm | ||
|
MatTek 96 (EVM-EL-03-02-05) |
Part # | Description | Pore size | Membrane |
|
|
CCI96-PET-0.4 | PermaCell Cell Culture Insert Plates, 96-well | 0.4µm | PET |
Sample plates
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This is a Corning #3378 24-well plate that may be used with the the EVM-EL-03-03-04.
The Corning Falcon 24-well plate is virtually identical to this one, and it may be used with the EVM-EL-03-03-03. |
This is a Millipore Multiscreen CaCo2 that may be used with the EVM-EL-03-03-02. | This is a Corning 3391 HTS Traswell 96 plate that may be used with the EVM-EL-03-03-05. |
EVM-EL-03-02-05
STX HTS EVOM™ Electrode for TEER in MatTek 96
- Compatible with EVOM Manual and EVOM3 meters
- For MatTek 96-well plates
- Exclusively compatible with Evom Manual
EVM-EL-03-03-02
STX HTS EVOM™ Electrode for EVOM Manual, use with Millipore Multiscreen HTS 96-Well Plate
- For Millipore Multiscreen HTS 96-Well Plate
- Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
- Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.
EVM-EL-03-03-03
STX HTS EVOM™ Electrode for EVOM Manual, use with Corning Falcon HTS Multi Well Insert System
- For Corning Falcon HTS Multi Well Insert System
- Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
- Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.
EVM-EL-03-03-04
STX HTS EVOM™ Electrode for EVOM Manual, use with Corning Costar HTS Transwell-24
- For Corning Costar HTS Transwell-24
- Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
- Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.
EVM-EL-03-03-05
STX HTS EVOM™ Electrode for EVOM Manual, use with Corning HTS 96-Well Plate
- For Corning HTS 96-Well Plate
- Mini XLR end connector for direct connection to EVOM Manual and EVOM3 meter
- Compatible with legacy meters including EVOM2 & Millicell ERS meters with the addition of connector EVM-AC-02-01-01.
EVM-AC-02-01-01
Mini XLR to RJ-11 Adapter Cable For Legacy TEER Meters
- Enables TEER electrodes for EVOM™ Manual to be used with legacy meters including EVOM2, EVOM, EVOMX, ERS, ERS2 TEER instruments
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