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Nicholas James, King's College London

Customer service was always quick and very helpful. Products all arrived quickly, were fully set up for us and have proved to be of good quality.
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Pressure Injection (Picoliter & Nanoliter Injectors)

Pressure Injection (Picoliter & Nanoliter Injectors)

WPI Microinjectors use carefully regulated air pres­sure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive “compensation” pressure to the injecting pipette between injection pulses to prevent fluid up­take through capillary action.

Timing, injection pressure and compensation pressure are adjusted inde­pen­dent­ly. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting. The injection pressure interval is triggered by using a foot switch or a computer-controlled TTL pulse (MICRO-ePUMP uses only a foot switch). 

The WPI Microinjectors are designed to inject very small quantities of fluids, such as drugs, into cells or small organelles. Pressure injection is an especially useful alternative to electroionophoresis, since it does not mandate the use of charged ions. Two different positive pressures may be applied–one for ejection at high pressure and a second, lower pressure to prevent back filling of the pipette by capillary action. 

The WPI Microinjectors offers separate regulated compensation (back filling prevention) and ejection pres-sures with a precision timing circuit that switches from injection pressure to compensation pressure automatical­ly.

WPI’s MICRO-ePORE Pinpoint Cell Penetrator technology is embedded inside the MICRO-ePUMP. When the researcher enables the The MICRO-ePORE, it delivers a highly localized voltage signal to a targeted injection site to facilitate penetration with minimal trauma. The researcher determines the amplitude and frequency of the signal that best suits the application. The signal originates in the control box, and it is transmitted through the electrode interface cable to the microelectrode holder. A silver wire is used to transmit the signal into the electrically conductive substance being injected. A reference electrode is used to place the media at 0.0 V potential with reference to the generated voltage.


Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps and MICRO-ePUMP use accurate timing and carefully regulated air pressures for securing cells and injecting. Injected volumes range from picoliters to nanoliters and are ideally suited for many applications including optogenetics and CRISPR-CAS9 studies.


MICRO-ePUMP

MICRO-ePUMP

MICRO-ePUMP Micro Injector with Cell Penetrator

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uPump

uPump

Microinjector with Internal Pressure Source

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PV850

PV850

Microinjector with External Pressure Source (pressure source not included)

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SYS-PV830

SYS-PV830

Pneumatic PicoPump with vacuum

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BRE	XenoWorks Digital Microinjector

BRE XenoWorks Digital Microinjector

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SYS-PV820

SYS-PV820

Pneumatic PicoPump

801963

801963

Miniature Vacuum Pump - 220V

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5430-ALL

5430-ALL

PicoNozzle Kit

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5430-10

5430-10

PicoNozzle Kit (for 1.0mm pipette)

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5430-12

5430-12

PicoNozzle Kit (for 1.2mm pipette) For use with the PicoPumps For use with 1.2mm pipettes Kit includes 5' of tubing


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5430-15

5430-15

PicoNozzle Kit (for 1.5 mm pipette) For use with the PicoPumps For use with 1.5 mm pipettes Kit includes 5' of tubing


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5430-20

5430-20

PicoNozzle Kit (for 2.0mm pipette) For use with the PicoPumps For use with 2.0 mm pipettes Kit includes 5' of tubing


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75122-110

75122-110

PicoNozzle Gasket, 1.0mm, Green, Pkg of 10 For use with the PV820, PV830, MMP, DMP


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75122-210

75122-210

PicoNozzle Gasket, 1.2mm, Black, Pkg of 10 For use with the PV820, PV830, MMP, DMP


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75122-310

75122-310

PicoNozzle Gasket, 1.5mm, Blue, Pkg of 10 For use with the PV820, PV830, MMP, DMP


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75122-410

75122-410

PicoNozzle Gasket, 1.65mm, Red, Pkg of 10 For use with the PV820, PV830, MMP, DMP

3316

3316

Replacement Input Kit for PicoPumps

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3260

3260

PicoPump Foot Switch


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75125-6

75125-6

Pipette holder for 5430-ALL. Package of 6. 
Washers and metal cap are NOT included


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2932

2932

Rack Mount Kit, 5 1/4-in. high


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2933

2933

Rack Mount Kit, 5 1/4-in. high


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MPH6R

MPH6R

Microelectrode Holder, Male Connector, Wire Half-Cell, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.


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MPH6S

MPH6S

Microelectrode Holder, Female Luer, Screw Cap. For use with these WPI Products: Piconozzle Kit (5430-XX). All holders are supplied for standard WPI single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters. Please specify diameter of glass you are using when placing your order.

300744

300744

ePUMP Capillary Kit

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Our Clients Include:

GlaxoSmithKline
University College London
Novartis
Imperial College
University of Cambridge
University of Oxford

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