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Benefits of NANOLITER2020 Injector (300704)

• Injections performed with mineral oil back-filled glass micropipettes, and the sample is front filled. This approach minimizes loss of any costly and scarce sample when a minute sample volume can be adequate. (See FAQ for details.)
• Control up to two pumps simultaneously
• Mounts easily on a stereotaxic frame or a micromanipulator
• Precise control over injection volumes (in the nanoliter to microliter range) and injection rates
• Simple to use touchscreen interface and graphical representation of volume status on MICRO2T controller
• LED run indicator offers a visual indication of connectivity
• Perform the injection with a foot switch (optional)
• Injector accepts a range of glass micropipettes (OD 1.1-1.5)
• NANOLITER2020 costs 15% less than the previous model (NL2010MC2T)!

Applications

Nanoliter to microliter injection into frogs (Xenopus oocytes), rats, mice, mosquitoes, shrimps, insects (e.g., Brown Planthopper), flies (e.g., Drosophila) and fish (Zebrafish) embryo using glass micropipettes

Please refer to the “References” tab for details.

NANOLITER2020 Improvements

• Connects directly to our MICRO2T SMARTouch™ controller, which can also be used with WPI’s UMP3 pump
• The injector head has a LED indicator which lights up bright red in RUN mode and dim red when connected to the controller. The LED indicator shows proper communication between the pump and controller.
• 14 mm OD glass micropipette is recommended for use with front green gasket. Use different OD fire-polished glass micropipettes by switching the front gasket (Green gasket for 1.1-1.15 mm OD glass, Black for 1.3-1.35 mm, Red for 1.5 mm).
• Improved precision by in-depth plunger displacement validation
• System is easier to use or operate
• Injector has a chic silver design

Components

NANOLITER2020 System includes all the components listed below.

NOTE: The 300704 Injector may also be purchase separately. 300704 does not include the Controller or any of the required accessories listed in the table below.

Two sample glass micropipettes are included. Use WPI-made glass micropipettes or use a glass capillary (504949) (included with NANOLITER2020) and a puller to make your own micropipettes.

FAQ

• What is the advantage of using oil back-filled glass micropipettes?

The term “back filling” refers to the process of filling the pipette from the large, non-pulled end.  “Front filling” is the term which is used to describe filling a micropipette through the small, pulled front end of the pipette. The glass micropipettes are first backfilled completely with mineral oil and secured to the NANOLITER Injector Head. Then, some mineral oil is dispensed through the tip. This creates the space and generates the pressure to front-fill samples through the tip. Front filling sample prevents the spillage or loss of costly or scarce sample involved with back-filling samples. When the sample volume is low, first back-filling glass micropipettes with oil and then front-filling the sample may be the only option.

• Can I use WPI’s old standard controller to run the 300704 Injector head?

WPI NANOLITER2020 Injector Head (300704) needs the MICRO2T controller (recommended). WPI’s MICRO4 controller may be used to control the pump. The 300704 Injector Head cannot be used with WPI’s old standard controller.

• Can I use same MICRO2T controller that I use with UMP3 pump? Do I need an additional adapter to use the 300704 pump with my existing MICRO2T controller?

Yes, the same MICRO2T controller can be used to control the UMP3 pump and the Nanoliter2010. No additional adapter is needed. 300704 connects directly to MICRO2T controller.

• Can I run the pump using a footswitch?

Yes, you can use the footswitch 13142. This is not included in the NANOLITER2020 system and is sold separately.

• What do I do when glass micropipettes come off after attaching or I am unable to front-fill a sample?

Odorless and colorless mineral oil is used for backfilling glass micropipettes. While the glass micropipette is installed and with gradual spillage over multiple uses, the gaskets become too slippery to hold glass micropipette in place. Use Kimwipes to soak up oil spillage inside the injector head, outside the glass micropipettes and to clean the gaskets. If needed, install a new set of gaskets to resolve the issue.

• I position the  O-rings  in the right order and orientation per your video but still don’t get an air tight seal .What can be done?  

If the rear seal is loose or mounted backwards then it can leak from air passage. This gasket needs to be tight on the plunger wire. If the pipette is pull tested, then it can be easily unseated from the middle gasket and leak by air infusion. The butt end of the glass needs to be smooth (and without any cracks in the glass) and firmly held down onto the middle seat while the collet is being screwed down. That plastic seat will deform a small amount and "mold" itself to the end of the glass. The fluids can also flow out on their own without any added back pressure even if there is a good seal, but that amount of mixing at a 10 um tip is very small. If either rear gasket allows for air flow, then it will leak.