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Millipore Millicell Compatibility

The EVOM™ Manual has been designed to improve workflow efficiency when working with Millipore MilliCell^® cell culture insert plates, delivering increased stability, and providing more repeatable measurements than older Trans Epithelial Electrical Resistance (TEER) meters including the Millipore MilliCell; ERS-2, Millipore MilliCell ERS and World Precision Instruments EVOM2.

For over 30 years, World Precision Instruments has been perfecting equipment for measuring Transepithelial Electrical Resistance (TEER). Researchers have relied on WPI's EVOM™ technology for the development of well over 16,000 peer-reviewed published research papers that use WPI TEER measurement meters. Here we compare the commonly used TEER meters in laboratories today. At a glance you can see the evolution of WPI's TEER technology and learn why it is the industry's standard TEER measurement methodology. Here's some of the benefits of upgrading to the EVOM™ Manual vs the MilliCell ERS voltohmmeter.

Comparison of EVOM™ Manual vs Millipore MilliCell ERS-2

 

	EVOM2 Epithelial Volt Ohm Meter	MilliCell® ERS-2 Voltohmmeter MERS00002	EVOM™ Manual TEER Measurement Meter EVM-MT-03-01
Tissue Sampling Frequency	12.5Hz	12.5Hz	12.5Hz
Resistance Range Modes	0 to 9,999 Ω	0 to 9,999 Ω	0 to 10,000 Ω 0 to 50,000 Ω 0 to 100,000 Ω AUTO
AC Square Wave Current	±10 µA	±10 µA	±10 µA for 10,000 Ω Range ± 4 µA for 50,000 Ω ± 2 µA for 100,000 Ω ± 2 μA, 4 μA, and 10 μA for AUTO mode
Electrode Connection	RJ-11 Connector STX Electrode Included	RJ-11 Connector STX Electrode Included	Mini XLR Connector Electrode Options Available, sold separately
Membrane Voltage Range	±200.0 mV Voltage Resolution= 0.1 mV	±200.0 mV Voltage Resolution= 0.1 mV	±200.0 mV Voltage Resolution= 0.1 mV
Unit Warmup Time	20 min.	20 min.	Ready for immediate use
Resistance Mode for TEER
PD (mV) Mode for Active Transport Studies
Compact, Lightweight & Portable
Non-Invasive Measurement with EVOM™ Technology
Smart Touchscreen Controls
Selectable Resistance Range
Auto Data Logging
Hands-Free Operation

Benefits of EVOM™ Manual vs Millipore MilliCell ERS-2

More Stability/Accurate Readings

EVOM™ Manual gives you quick, easy, and reliable readings due to its fast stabilization, automatic twenty times sampling average, and low noise design. An overrange display feature eliminates erroneous readings.

Avoid the Need to Track Data by Hand

Millipore MilliCell ERS-2 requires you to log data by hand which is cumbersome and subject to transcription errors. EVOM™ Manual automatically captures the resistance or voltage readings to a USB drive in CSV format for easy access to your data, including date and time stamps on saved data. You can eliminate recording errors and reduce experimental processing time.

Variety of Measurement Ranges

Millipore MilliCell ERS-2 meters are limited to a single measurement range, but EVOM™ Manual allows you to select different ranges or can automatically detect the target range. Whether you are measuring a leaky cell type with low resistances or cell types for which barrier function is critical and resistance is higher, like blood brain barrier (BBB), the EVOM™ Manual can auto select. The auto-ranging resistance feature allows for fast resistance measurements. The EVOM™ Manual now has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistance ranges up to 100K Ω, making the EVOM™ Manual suitable for a variety of cell and tissue culture types (skin, intestine), complex 2-D (co-culture) or 3-D model (BBB) applications.

Hands Free Recording

With EVOM™ Manual you can streamline your process with a footswitch for hands-free recording, making sample analysis even faster. Data is recorded automatically, every time you tap the footswitch. You also minimize the chance of sample contamination when you limit hand movement during operation.

Touchscreen Display

EVOM™ Manual has an intuitive touchscreen controller with and easy-to-navigate menu and a graphical display showing your data for vital feedback during experimental measurements. The Millipore MilliCell ERS-2 show a simple numerical reading. With the EVOM™ Manual, you see the data for all the plate samples.

Millipore Plate Compatibility With EVOM™ Manual

The EVOM™ Manual requires an electrode for use. Choose an electrode suitable for your application.

Millicell Standing Inserts

Millipore Plate	Compatible TEER Electrode	Device Configuration	Description
PSHT004 PSHT004R1 PSHT004R5 PSHT004S5	STX HTS	96-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 0.4 µm Filtration Area: 0.11 cm²
PSRP004 PSRP004R1 PSRP004R5	STX HTS	96-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 1.0 µm Filtration Area: 0.11 cm²
PIHP01250	STX4 Endohm-6	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 0.4 µm Filtration Area: 0.6 cm²
PSHT010R PSHT010R1 PSHT010R5	STX4	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 0.4 µm Filtration Area: 0.7 cm²
PICM01250	STX4 Endohm-6	24-well plate	Chemistry & Media: Hydrophilic Polytetrafluoroethylene (PTFE) / Biopore Pore Size: 0.4 µm Filtration Area: 0.6 cm²
PIHA01250	STX4 Endohm-6	24-well plate	Chemistry & Media: Mixed Cellulose Esters (MCE) / MF-Millipore Pore Size: 0.45 µm Filtration Area: 0.6 cm²
PSRP010 PSRP010R1 PSRP010R5	STX4	24-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 1.0 µm Filtration Area: 0.7 cm²
PITP01250	STX4 Endohm-6	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 3.0 µm Filtration Area: 0.6 cm²
PSST010R PSST010R1 PSST010R5	STX4	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 3.0 µm Filtration Area: 0.7 cm²
PSMT010R PSMT010R1 PSMT010R5	STX4	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 5.0 µm Filtration Area: 0.7 cm²
PI8P01250	STX4	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 8.0 µm Filtration Area: 0.6 cm²
PSET010 PSET010R1 PSET010R5	STX4	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 8.0 µm Filtration Area: 0.7 cm²
PIXP01250	STX4 Endohm-6	24-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 12.0 µm Filtration Area: 0.6 cm²
PICM0RG50	Endohm-24	6-well plate	Chemistry & Media: Hydrophilic Polytetrafluoroethylene (PTFE) / Biopore Pore Size: 0.4 µm Filtration Area: 4.2 cm²
PIHP03050	Endohm-24	6-well plate	Chemistry & Media: Polycarbonate (PC) / Isopore Pore Size: 0.4 µm Filtration Area: 4.2 cm²
PICM03050	Endohm-24	6-well plate	Chemistry & Media: Hydrophilic Polytetrafluoroethylene (PTFE) / Biopore Pore Size: 0.4 µm Filtration Area: 4.2 cm²
PIHA03050	Endohm-24	6-well plate	Chemistry & Media: Mixed Cellulose Esters (MCE) / MF-Millipore Pore Size: 0.45 µm Filtration Area: 4.2 cm²

 

Millicell Hanging Inserts

Millipore Plate	Compatible TEER Electrode	Device Configuration	Description
PTHT24H48 PCHT24H48	Endohm-6	24-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 0.4 µm Filtration Area: 0.3 cm²
PTRP24H48 PLRP24H48	Endohm-6	24-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 1.0 µm Filtration Area: 0.3 cm²
PTSP24H48 PCSP24H48	Endohm-6	24-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 3.0 µm Filtration Area: 0.3 cm²
PTMP24H48	Endohm-6	24-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 5.0 µm Filtration Area: 0.3 cm²
PTEP24H48	Endohm-6	24-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 8.0 µm Filtration Area: 0.3 cm²
PTHT12H48	EndOhm-12	12-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 0.4 µm Filtration Area: 1.1 cm²
PTRP12H48	EndOhm-12	12-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 1.0 µm Filtration Area: 1.1 cm²
PTSP12H48	EndOhm-12	12-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 3.0 µm Filtration Area: 1.1 cm²
PTMP12H48	EndOhm-12	12-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 5.0 µm Filtration Area: 1.1 cm²
PTEP12H48	EndOhm-12	12-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 8.0 µm Filtration Area: 1.1 cm²
PTHT06H48	EndOhm-24	6-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 0.4 µm Filtration Area: 4.5 cm²
PTRP06H48	EndOhm-24	6-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 1.0 µm Filtration Area: 4.5 cm²
PTSP06H48	EndOhm-24	6-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 3.0 µm Filtration Area: 4.5 cm²
PTMP06H48	EndOhm-24	6-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 5.0 µm Filtration Area: 4.5 cm²
PTEP06H48	EndOhm-24	6-well plate	Chemistry: Polyethylene Terephthalate (PET) Pore Size: 8.0 µm Filtration Area: 4.5 cm²

 

EVOM™ Manual Electrodes

Cell Culture Insert/Transwell Type	Electrode	Advantages	Disadvantages
Removable 24-well	STX4	No need to transfer inserts. Take measurments in the plate.
Removable 24-well	EndOhm-6	Most accurate mode of measurement, because of the larger electrode surface area and the concentric positioning of the electrodes.	Need to transfer inserts into the EndOhm chamber for making measurements.
Removable 12-well	EndOhm-12
Removable 6-well	EndOhm-24
24- or 96-well High Throughput Screening (HTS) Plates	STX HTS	Fits perfectly in the measurment plate. Choose the option based on your specific plate.

While the EVOM™ Manual operates on the same proven EVOM™ technology as EVOM2 and Millipore MilliCell ERS-2, significant hardware and software upgrades provide exceptional new functionality to improve reading reliability and workflow efficiency. Give us a call today to talk with an application specialist.

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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