Features ● Unique, diffusion grid, combined with air input and return vents provide an air flow pattern for consistent, even heating, with no hot or cold spots in the chamber External heater that can be placed far enough from the system to eliminate electrical and vibrational interference from the heater High degree of temperature precision and stability Minimal focal drift after equilibrium is achieved– Accuracy ±0.1ºC at the sample itself, and 0.2ºC across the microscope stage (allowing for uniform heating of multiwell dishes) Airflow pattern and temperature uniformity eliminate dramatic changes in environmental temperature when the incubator door opens Ergonomic design for ease of use– The focus and x/y stage controls are outside of the incubator itself. Large doors allow easy access to the specimen and small ones for cords, tubing, etc. Precision, shielded temperature probe Simple, one person setup of the system ● ● ● Ordering an Environmental Chamber Acrylic enclosures are essentially custom-built. When ordering a system, you will need to provide the following information: Microscope Perfect Focus Right Port Tirf Tilting Head Emission Cells Stage Camera Analyzer White Light Tirf Filter Wheels: Dual Lamphouse 35mm/60mm Coverslips Stage-Up Left Port Fluor Attachment Binocular D Head Excitation Transmitted Light Shutter Wells ● ● MICROSCOPES, CAMERAS ● ● Live Cell Microscopy System Components This unique, acrylic Live Cell Imaging chamber, combined with an Environmental Control Unit (ECU) and an AirTherm controller, ensured precision control of your incubator environment. The ECU comes in four varieties so you have all the control you require. With the ECU-H5, you can control air flow and heating. In addition to air flow and temperature control, the ECU-HC and ECU-HCP let you control the CO2 level. One has an internal sensor, and the other has a remote sensor that can be positioned inside the microscope chamber. Finally, the ECU-HOC adds control of the O2 level, which is accomplished by displacing the oxygen with nitrogen. The first three ECU units are capable of controlling a simple, external heater, like the AirTherm Satelite (AirTherm-SAT) or a microscope lens warmer. The AirTherm-SMT can monitor and control both temperature and humidity inside the microscope chamber. Fig. 1–Single air input and no venting causes random air flow in a traditional incubator. Fig. 2–Hot and cold spots result from inconsistent flow. Consistent Air Flow Air flow affects the temperature uniformity of incubators. The red arrows on Fig. 1 and Fig. 3 indicate air flow. The Live Cell Microscope Incubator uses a diffuser grid and proper venting to insure consistent air flow. Traditional incubators with poor air flow suffer with hot and cold spots in the incubator, as seen in thermal images (Fig. 2 and Fig. 4). Warmer temperatures are indicated by red and cooler temperatures by blue. Fig. 3–A diffusion grid with air input and exhaust vents yields consistent air flow. Fig. 4–Consistent air flow means uniform heating. IN VIVO FULL CHAMBER SYSTEM WITH ECU CONTROLLER & NEW AIRTHERM-SAT/SMT All Systems Include: Proprietary Humidification Module, Stage Adaptor, Stage Dish with Optical Grade Glass. IV-100SMT IV-200ECU IV-200-OX IV-300ECU IV-CUST Environmental Chamber, Heat controller. Requires pre-mixed 5% CO2 gas supply Environmental Chamber, Heat & CO2 controller. Requires 100% CO2 & ambient air supplies. . Microscope Environmental Chamber, Heat Controller, CO2 and O2 Controller. Environmental Chamber, Heat & CO2 controller. Requires 100% CO2 & ambient air supplies. Features a CO2 probe to detect concentration at the sample. Custom Design includes but not limited to Tilting Heads, Black Chamber, Confocals, Camera on Right, HMX Lamphouse, and Manipulators. Fee added for design not included in standard system UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com 149