Nitric Oxide Sensor Guide All WPI NO sensors are 100% compatible with ISO-NO Mark II (NOMK2), APOLLO 4000, APOLLO 1000, and TBR4100 Free Radical Analyzer. APPLICATIONS SENSOR SENSOR DIAMETER RESPONSE TIME (with NOMK2) LOWEST DETECTION LIMIT TEMPERATURE SENSITIVITY DRIFT SENSITIVITY PHYSIOLOGICAL INTERFERENCE in vivo ISO-NOPF 100, 200, or 500 µm < 5 sec 0 .2 nM some none 10 pA/nM none Cell Cultures, NO2 , NO3, ISO-NOP 2 mm < 5 sec 1 nM yes none 2 pA/nM none Tissue Bath ISO-NOP30L 30 micron < 3 sec 1 nM yes none 1 .4 pA/nM none Tissue Bath ISO-NOP70L 70 micron < 3 sec 1 nM yes none 1 .4 pA/nM none Microvessels ISO-NOP30 30 micron < 3 sec 1 nM yes none 1 .4 pA/nM none Microvessels ISO-NOP007 7 micron < 3 sec 0 .5 nM yes none 1 pA/nM none Single Cell ISO-NOPNM 100 nm < 3 sec 0 .5 nM some none 0 .5 pA/nM none Selectivity of WPI’s NO sensors BIOSENSING The ideal NO sensor should be insensitive to other reactive species likely to be present within the measurement environment. The conventional Nafion coated carbon fiber NO sensor exhibits a large response to such species. WPI’s unique NO sensor technology utilizes a novel surface membrane which amplifies the response to NO while eliminating responses to a vast range of reactive species, including nitrite, absorbic acid, hydrogen peroxide, catecolamines, and much more. oxide probe – ideal for cell cultures, cell suspensions and many other applications ISO-NOP – The original nitric – NO3 and NO– Detection 2 A simple change in experimental protocol will enable the ISO-NOP to be conveniently used for indirect rapid accurate determination of – – nitrite (NO 2) and nitrate (NO 3) concentration in samples. Using this method a detection – – limit for NO 2 or NO 3 as low as 1 nM is routinely possible. Abdominal X-ray showing the appratus consisting of two customized ISO-NOP nitric oxide probes, 4-channel pH catheter, and Teflon nasogastric tube. (Courtesy Prof. K.E.L. McColl, University Department of Medicine and Therapeutics, Western Infirmary, Glasgow, Scotland.) Iijima, K., et al. Gastroenterology 2002: 122: 1248-1257. The ISO-NOP is a popular, robust and high performance sensor encased within a 2 mm diameter disposable stainless steel protective sleeve. The tip of the sleeve is covered with a NO-selective membrane. Replacement membrane sleeves can be purchased separately (WPI #5436) and require an internal electrolyte (WPI #7325). ISO-NOP 5435 5436 7325 7521 5399 7357 Replacement 2 mm shielded sensor and cable ISONOP Startup Kit (recommended with first purchase) Replacement Sleeve Kit for 2 mm sensor, pkg of 4 ISO-NO Electrolyte (10 mL) ISO-NO Electrolyte, CO2-insensitive (10 mL) T-Adapter Kit (pkg of 3) for ISO-NOP Nitrite Standard Solution, 1 gram/liter (100mL) making it indisputably the smallest and most sensitive NO sensor in the world! The ISO-NOPNM is based on a novel design in which an electrochemically “activated” Ag/AgCl composite graphite nanofibre is used as Reference Electrode Sensor Body 70mm long Glass tip tapers to 7µm Ø NO-sensing element. The surface of the the Fiber ~100µm Tip Sensing Element NanoSensor is then modified using a unique tapers to multi-layered NO-selective membrane. ~100nm tip Figure at right illustrates the response of the ISO-NOPNM following successive additions of nanomolar concentrations of NO. The ~100– ~2mm 200µm ultra-low noise of the ISO-NOPNM (0.5 pA) enables a detection limit of just 0.5 nM NO. Schematic drawing of the new integrated NO nanoThe response time of ISO-NOPNM is less sensor. (US Patent Pending) than 3 seconds. smallest nitric oxide NanoSensor, designed for measurement of NO at the cellular level. The ISO-NOPNM NanoSensor has a tip diameter of just 100 nm (0.1 µm) and a detection limit for NO of less than 0.5 nM — ISO-NOPNM 91580 SNAP50 ISO-NOPNM – The world’s 800 Current / pA Current / pA 600 400 200 0 0 y = 0.5968x + 6.4967 R2 = 0.9973 300 600 900 1200 Concentration / nM Time / S Amperometric response of the NO nanosensor (ISONOPNM) to the successive additions of 2nM, 4 nM, 8 nM NO into 0.1 M PBS (pH=7.4). 100 nm NanoSensor, pkg of 3 (requires cable #91580) Microsensor Adapter Cable SNAP, 50 mg vial 52 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com