TEER measurements in High Throughput STX100 Series Electrodes ● Designed for 24-well HTS plate (Corning Costar and BD Falcon) and with 96-well plates (Millipore and BD Falcon) ● Improved accuracy down to 5 Ohm ● Sterilized with EtO, alcohol or bactericide EPITHELIAL PHYSIOLOGY With the development of a High Throughput Screening (HTS) protocol for faster drug discovery, a new line of cell culture filter plates have been introduced by several major cell culture insert manufacturers. These HTS plates normally have either 24 or 96 individual cell culture inserts “bonded” together as one plate so that it can be handled by a robot apparatus. In response to these developments, WPI has developed an automatic REMS system and a manual electrode, STX100, for TEER measurements using HTS plates. STX100’s design is based on the same reliable design principle as the universally used STX2 electrode, with several important modifications. The size of the electrode tip has been reduced to 1.5 mm to facilitate positioning through the narrower slit of the HTS plate. The STX100 electrode itself is constructed using a stronger material for higher durability and maximum usage applications. The bottom section of the electrode is shaped to fit neatly into the “keyhole” shaped filter well. This enables the STX100 electrode to produce increased accuracy and reproducibility of TEER readings (±5Ω) compared to the standard STX2. Several versions of STX100 are available, designed to fit the Corning Costar 24-well HTS plate, the Falcon 24 well HTS plate, and the Millipore Multiscreen CaCo 96-well plate. Measurement can be directly performed when the HTS plate is in either a common or divided tray, reducing the possibility of contamination as well as mechanical damage to the cultured cells. STX100C STX100F STX100M STX100C96 STX100 for Corning Costar HTS Transwell-24 STX100 for BD Falcon HTS Multiwell Insert System STX100 for Millipore MultiscreenTM HTS 96-Well Plate STX100 for Corning HTS 96-Well Plate OPTIONAL ACCESSORIES 13685 Modular Cable, 7 ft 13347 Chart Recorder Adapter 2851 Standard BNC Cable, 5’2” 500184 Standard BNC Cable, 10 ft (3m) CaliCell™ Cell culture cups with synthetic membrane for testing STX electrodes, Endohm and Ussing chambers It takes a long time and a lot of work to grow a batch of cells, so you will want to make certain that your test apparatus is functioning properly. The CaliCellTM provides a quick and positive way to test STX electrodes, EVOMs, Endohm, and Ussing chamber. The CaliCellTM is a major improvement in TEER electrode calibration. Its membrane makes use of our unique electric current constriction technology to produce resistance readings comparable to those obtained with real cell cultures. The CaliCellTM does not have to be refrigerated, and can be cleaned and sterilized with alcohol. Readings will not drift over time as long as the unit is kept in good physical condition. CALICELL-12 12 mm Calibration Cell for Endohm-6/Endohm-12 82 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com