Records redox currents with carbon fiber microelectrodes Advanced Neurotransmitter Detection Measures oxidizable compounds such as catecholamines (epinephrine, norepinephrine, dopamine), indolamines (serotonin, melatonin), ascorbic acid and Fe (II) with exquisite sensitivity, low noise and site specificity 1-picoampere to 2 microamperes. The built-in carbon electrode activation feature allows the easy renewal of electrode sensitivity. In addition, MicroC features a low-pass filter and the option of applying DC potential externally. A wide range of compounds can be detected: dopamine, epinephrine, norepinephrine, serotonin, etc. Other compounds, such as glutamate, glucose, acetylcholine and alcohol, can also be detected with MicroC using enzyme-modified biosensors. See Application Notes, “Carbon Fiber Microelectrodes”, available as a PDF file which may be downloaded from WPI”s web site. BIOSENSING Use with WPI electrodes — see next page MicroC, WPI’s low cost and elegant instrument for electrochemical detection using carbon microelectrodes, will record the presence and concentration of oxidizable biological compounds in vivo and in vitro. It also features inherently low noise and a sensitivity of 1 millivolt per picoampere of oxidation current. Response time to quantal catecholamine release is less than 1 millisecond. When used with carbon fiber microelectrodes, redox current can be recorded over a range of The MicroC Potentiostat is supplied with a carbon electrode probe, with 5 feet triax cable, which accepts 0.79 mm connector pin, and a reference electrode with a 4 mm Ag/AgCl half cell (see page 121). For applications where smaller half cells are needed, please call WPI for more information. References G. A. Gerhardt, “Nafion-coated electrodes with high selectivity for CNS electrochemistry” Brain Research, 290: 390-395 (1984). R. M. Wightman, et al., “Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.” Pro. Nat’l Acad. of Sci. 88: 10754-58, (1991). Z. Zhou and S. Misler, “Action Potential-induced Quantal Secretion of Catecholamines from Rat Adrenal Chromaffin Cells”, J. Biol. Chem. 270; 3498-3505, (1995). MICROC SPECIFICATIONS METHOD APPLIED POTENTIAL CURRENT RANGES BANDWIDTH NOISE DISPLAY RECORDER OUTPUT RISE TIME POWER BATTERY LIFE SHIPPING WEIGHT 2 electrode, DC potentiostat 0.65 V, variable ± 2.5 V 2000 pA, 20 nA, 200 nA, 2 µA 1.67 Hz, 167-1000 Hz < 1 pA 31⁄2-digit LCD display, ±2 V ± 4.5 volts < 1 millisecond Six 1.5 volt alkaline batteries (included) > 1000 hours, est. 4 lb (1.8 kg) ELECTRODE PROBE/CABLE LENGTH Triax shielded, 5 feet SYS-MICROC Potentiostat MICROCP Replacement Probe for MicroC OPTIONAL ACCESSORIES 300305 ATP Adapter (0.031" pin to 1 mm socket) 58 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com