Dri-Ref™ Reference Electrodes R DRI DR EF-4 50 Although the internal filling solution contains KCI, the low fluid leakage means Dri-Ref may be used in combination with ion selective electrodes, including those for K+ and Cl-, without significant contamination from the reference electrode. The Dri-Ref electrodes are chemically resistant to strong acids and alkalines. Dri-Ref electrodes are not suitable for use in organic solvents. In addition, the long, thin FLEXREF may be easily manipulated to accommodate a difficult experimental setup. F-5 IRE EF-2 DRIR FL SUPER-Dri-Ref With a diameter of 2-mm, SUPER-Dri-Ref does not leak electrolyte at all. Exhibiting the electrical stability of a classic flowing junction reference cell, this electrode exhibits low resistance and a stable half-cell potential essentially independent of sample electrolyte concentration. SUPER-Dri-Ref is ideal for small volume and low salt concentration measurements. EF EXR SUP ER -Re -Dri f, SD R2 E DRIR F-L Micro-Reference Electrode SH Only 450 µm in diameter and 1 inch long, WPI’s new DRIREF-450 reference electrode can be used along with other sensors in space-restricted areas and very small sample volumes. BIOSENSING R DRI DRI EF-5 - Luer-Tip Reference The male luer fitting at the front of the DRIREF-L allows it to be easily connected to a female luer port (see WPI’s luer fittings kit, page 138) to form a tight seal — a very convenient installation for a flow-through system. REF 2SH Dri-Ref™ reference electrodes were developed by WPI to have extremely low electrolyte leakage properties, hence the name “Dri-Ref”. In addition to this key feature, these electrodes exhibit stable and reproducible potential and low resistance. Stored in KCl when not in use, they have a long life expectancy. FLEXREF DRIREF-2 DRIREF-2SH DRIREF-5 DRIREF-5SH SDR2 DRIREF-450 DRIREF-L Flexible Dri-Ref, 1.5 mm diam. Dri-Ref, 2 mm diam. Dri-Ref, 2 mm diam. (Short) Dri-Ref, 4.7 mm diam. Dri-Ref, 4.7 mm diam. (Short) SUPER-Dri-Ref, 2 mm diam. Micro-Dri-Ref, 450 µm diam. Reference Electrode with Luer Tip DRIREF-450 LENGTH 2.54 cm DIAMETER 450 µm CONSTRUCTION Coated Glass LEAD LENGTH 30 in. (76 cm) CONNECTOR 2 mm pin RESISTANCE (typical) <5KΩ FILLING SOLUTION KCl ELECTROLYTE LEAKAGE (mL/hr) — DRIREF-5 9 cm 4.7 mm Epoxy 30 in. (76 cm) 2 mm pin ~500 Ω KCl ~7.4×10-7 DRI-REF SPECIFICATIONS DRIREF-2 13 cm 2 mm Isoplast™ 30 in. (76 cm) 2 mm pin ~2.7 KΩ KCl ~5.7×10-8 FLEXREF 13 cm 1.5 mm Teflon™ 30 in. (76 cm) 2 mm pin ~2.7 KΩ KCl ~5.7×10-8 SDR2 9 cm 2 mm PVC 30 in. (76 cm) 2 mm pin < 5 KΩ KCl — DRIREF-L 7.5 cm Standard Luer Polypropylene 30 in. (76 cm) 2 mm pin ~500 Ω KCl ~7.4×10-7 DRIREF-5SH 3.5 cm 4.7 mm Epoxy 30 in. (76 cm) 2 mm pin ~500 Ω KCl ~7.4×10-7 DRIREF-2SH 2 cm 2 mm Isoplast™ 30 in. (76 cm) 2 mm pin ~2.7 KΩ KCl ~5.7×10-8 Isoplast is a trade mark of Dow Chemical. Teflon is a trade mark of DuPont. Calcium Calibration Solutions CALBUF-1 For use with calcium electrodes A set of eight calcium buffers covering the range of concentration from 10-1 to 10-8 M Ca++. Each buffer contains 20 mL of solution and enough potassium chloride to set the ionic strength to 0.1 M. Limited shelf life; use within 30 days. Concentration: 1x10-1, 1x10-2, 1x10-3, 1x10-4, 1x10-5, 1x10-6, 1x10-7, 1x10-8 M at 20°C. Limited shelf life; use within 30 days. CALBUF-1 Kit of 8 Calcium Buffer Solutions CALBUF-2 For use with calcium fluorescent indicators CALBUF-2 is especially suitable for calibrating fluorescent Ca++ indicators. It provides eleven buffer standards in the 10-4 to 10-8 M Ca++ range, whereas other commonly used fluorescent Ca++ indicators have the apparent Kd in the range of 100 to 300 nM. As with any ionic sensitive indicator, the sensitivity range of these indicators is about 1.0 log unit above and below the Kd. CALBUF-2 provides seven calibration points in this sensitivity range. It has an osmolarity of 0.305, which is isotonic with most mammalian cells. Concentration: 1x10-8, 4x10-8, 1x10-7, 2.5x10-7, 5x10-7, 7.5x10-7, 1x10-6, 4x10-6, 1x10-5, 4x10-5, and 1x10-4 M at 20°C. Ionic strength: 0.150 M. 11 bottles, 20 mL each. Limited shelf life; use within 30 days. CALBUF-2 Kit of 11 Calcium Buffer Solutions UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com 65