Duo 773 Dual Microprobe System 2-channel intracellular amplifier AMPLIFIERS, ELECTROMETERS For intracellular dual or differential studies, the Duo773 has separate negative capacity controls and built-in active filtering that allows the precise balancing of time constants for artifact-free differential measurement. Comes complete with two probe headstages, 1015 Ohms & 1011 Ohms probes to monitor signals from ion-specific micro-electrodes as well as KCl-filled electrodes. * Although injected currents are “constant,” the maximum current in a given situation will always be limited by the system compliance of 10 V. **The 712P headstage may be used on either A or B channels, however Current Injection specifications do not apply when used on channel A. The 715P headstage may not be used on the B channel. DUO 773 SPECIFICATIONS HEADSTAGE (PROBE) ACTIVE PROBE INPUT IMPEDANCE GAIN OUTPUT RESISTANCE OUTPUT VOLTAGE RANGE MAXIMUM INPUT VOLTAGE PROBE LEAKAGE CURRENT DC POSITION ADJUST RANGE ELECTRODE RESISTANCE TEST CURRENT INPUT CAPACITY COMPENSATION NOISE Input shorted 20 MΩ carbon resistor RISE TIME 10-90% direct input small signal 10-90% through 20 MΩ (-C “on”) CURRENT INJECTION Internal DC Current Externally commanded Current External current command factor Current monitor Compliance Bridge balance Bridge amplifier gain LOW PASS FILTER METER SECTION Display Ranges Accuracy and resolution DIMENSIONS Instrument Probe POWER SHIPPING WEIGHT CERTIFICATION 712P (red, port “B”) >1011Ω x1, x10 100 Ω ±10 V ±15 V 5 X 10-12 A ± 300 mV 1 nA +10 to -50 pF <50 µV p-p 10kHz bandwidth <200 µV p-p 10kHz bandwidth 715P (blue, port “A”) 1015Ω x1 100 Ω ± 10V ±15 V 10-14 A ± 300 mV 1 pA, 1 nA selectable 0 to -10 pF <50µV p-p 10kHz bandwidth <200µV p-p 10kHz bandwidth References L. Pluja (2000) “Electrical and mechanical effects of vasoactive intestinal peptide and pituitary adenylate cyclase-activating peptide in the rat colon involve different mechanisms.” European Journal of Pharmacology 389, 217224. G. X. Wang, X. B. Zhou, et al. (2000) “Effects of mitoxantrone on excitation-contraction coupling in guinea pig ventricular myocytes.” Journal of Pharmacology and Experimental Therapeutics 293, 2, 501-508. S. Tsuruoka (2000) “Acute effect of cadmiummetallothionein on glucose and amino acid transport across the apical membrane of the rabbit proximal tubule perfused in vitro.” Journal of Pharmacology and Experimental Therapeutics 292, 2, 769-777. 1 µs, typical 25 µs, typical (712P only)** ± 50 nA low range, ± 500 nA high range ± 500nA low range, ± 5 µA high range 20 mV/nA low range, 2 mV/nA high range 100 mV/nA low range, 10 mV/nA high range 3V low range, 10V high range 0-100 MΩ, 0-1000 MΩ x 10, x 50 40 dB/decade, continuously variable 1-30 kHz 3.5-digit LED 200 mV, 2000 mV, 20 V, 200 nA, 2000 nA 1 digit 17 x 5.25 x 10 in. (43 x 13 x 25 cm) Diameter: 12 mm Length: 34 mm 95-135 V or 220-240 V, 50/60 Hz 15 lb (7 kg) CE, CSA 70 ORDER TOLL-FREE: 866-606-1974 (U.S. only) World Precision Instruments • Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com • Internet: www.wpiinc.com