Nanoliter Injector NANOLITER 2010 For oocyte injection and applications in the 2 to 70 nanoliter range WPI’s microprocessor-controlled Nanoliter 2010 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/ sec). Maximum fluid ejection is 5 µL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5 mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment. Included: 1 vial 3.5 in. capillaries (300); replacement “O” rings; Allen wrench; MicroFilTM MF34G backfilling needle; and two sample µTip™ prepulled micropipettes. PUMPS, MICROINJECTION Micromanipulator not included. Smart Controller: Micro4, an optional microprocessor-based controller, can provide an “intelligent” and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane keypad and LCD display. From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device’s “non-volatile” memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller Optional smart controller for for “hands free” start/stop Nanoliter 2010 operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs. WPI recommends the following equipment for a complete sys tem,: PZMIII stereo microscope • MMJ joystick micromanipulator • M10 magnetic base • µTip™ micropipettes. NANOLITER 2010 SPECIFICATIONS INJECTION VOLUME REMOTE CONTROL GLASS OD GLASS ID STEP INJECTION SPEED Slow Fast FILL SPEED Slow Fast EMPTY SPEED VARIABLE VOLUME RANGE SMALLEST VOLUME TO CHANGE VOLUME INJECTIONS PER FILLING, MAX. SHIPPING WEIGHT Variable Yes 1.14 mm 0.5 mm 12.7 µ/step 23 nL/sec 46 nL/sec 23 nL/sec 46 nL/sec 92 nL/sec 2.3 - 69.0 nL 2.3 nL Set switch 100 injections 3 lb. (1.1 kg) NANOLITER2010 Nanoliter 2010 NL2010MC4 Nanoliter Injector & Micro4 Controller (small controller not included) OPTIONAL ACCESSORIES 13142 Footswitch for Nanoliter 2010 15867 Footswitch for Micro4 Controller 4878 Replacement 3.5-in. glass capillaries (300) 4879 Replacement 7-in. glass capillaries (300) TIP10XV119 Micropipettes for Nanoliter Injector (10) SYS-MICRO4 Micro4 Controller, Four-Channel 300033 Adapter for Micro4 300521 Replacement O-rings (five) 500778 Replacement Nanoliter Injector Universal Adapter 500299 Pistons, 5-pack UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com 177