Biofluorometer Now more reliable, simplified and affordable The new SI-BF100 is an LED-based fluorometer for life science applications. It is ideally suited for ratiometric calcium detection (FURA-2) and ATPase detection (via NADH fluorescence). With up to seven LED modules (wavelengths), the SI-BF100 covers many fluorometric applications in neuroscience and cell biology. Recent advancements in optics and LED technology simplify ratiometric calcium imaging, making this equipment more affordable. A breakthrough in WPI patented technology allows the SI-BF100 to use wavelengths below 380nm and produce more light in those spectra. This technology significantly cuts the cost of photometric calcium imaging without sacrificing resolution or quality. LED light sources require less power, give off less heat and are more compact and affordable Sampling rates up to 1kHz (1,000 ratios/ second maximum). At lower speeds, signal averaging is used for noise reduction. Two auto ranging photomultiplier inputs allow you to monitor multiple wavelengths from a single emission output that can be comprised of any wavelength of light for which an LED module is available Using a separate reference channel, ultrastable, continuous ratio calculations automatically compensate for LED intensity drift. This ensures less noise and produces more accurate measurements. Application-specific probes are available for existing tissue baths and cuvette systems. Ratio noise is <0.05 peak to peak, drift is less than 0.1 unit/hour The warm up time of less than one minute is a dramatic improvement over the common 20–60 minutes required by xenon or mercury light sources Replace the emission filter easily or change the LED modules to transform the SI-BF100 into a general purpose fluorometer for many other applications CALL FOR APPLICATION How it Works Up till now, calcium imaging systems have been required to compensate for errors and noise introduced by the complexity of their design. The systems require mechanical filters and use expensive xenon or mercury light sources. The beauty of the SI-BF100 is its simplicity. The elegance of its design reduces the noise introduced into the system and the errors inherent in traditional systems. e Ratiom m & Calciuase ATP output to one or two photomultiplier inputs on the front of the SI-BF100, which are independently filtered for specific wavelengths. This design allows you to monitor multiple wavelengths from a single emission output. The LED light source in the WPI design makes this ratiometric fluorometer more compact, energy efficient and affordable. As added benefits, the low-power light source produces much less heat, and it warms up in less than one minute! t for: Perfec tric SPEC TROS CO PY Monochromatic LED light sources using WPI patented technology eliminate the need for complex and expensive white light This incredible design is not limited to calcium sources and filter wheels. imaging either. By simply replacing Because the LED the 510nm emission filters modules can Unique fiber optic coupling probe allows in front of the for highly efficient transfer of light and be pulsed, photomultipliers ease of placement. Custom probes fit sampling with the desired your existing systems. frequencies up wavelength filters, to 1,000 cycles per your SI-BF100 becomes second are possible. a general purpose fluorometer for any The LED light source emits specific excitation frequencies which travel through the probe. The excitation light can be comprised of any wavelength of light for which an LED module is available. The probe returns a single emission application you can imagine. Changing a filter involves removing the two screws that hold the filter carriage on the face of the SI-BF100, swapping the filter and reinstalling the integral SMA/filter carriage. SI-BF-100 Biofluorometer OPTIONAL COMPONENTS M3301 Manual Manipulator for securing the probe M10 Magnetic Base SI-BF100 Specifications FIBER OPTIC LIGHT INPUT/OUTPUT . . . . . . . . . . .SMA terminated BANDWIDTH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1000 ratios/second RATIO NOISE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .< 0.1 peak to peak ANALOG OUTPUT RANGE . . . . . . . . . . . . . . . . . . .0–10V (continuous, equivalent to a ratio 0–10) ANALOG OUTPUT IMPEDANCE . . . . . . . . . . . . . . .100W POWER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12VDC, 0.5A, (universal power supply, 110/240VAC) WARM UP TIME . . . . . . . . . . . . . . . . . . . . . . . . . . . . .<1 minute DIMENSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3.5"H x 17"W x 13"D (88 x 431 x 330 mm) 190 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com