OxyMini & OxyMicro A new generation of fiber optic oxygen sensors based on luminescence lifetime The OxyMini system is optimized for process control and biotechnology applications. The OxyMicro is designed for biological research applications including implanting into tissues, cell cultures, profiling of biofilms and sediment related bioassays. The measurement principle of the sensor system is based on the detection of oxygen concentration as a function of luminescence lifetime either in dissolved or gaseous phase environments. MicroFlow The MicroFlow fiber optic oxygen sensor (WPI #501657) is a miniaturized fiber optic chemical sensor optimized for fast response time (t90 < 1 sec in gases, < 5 sec in liquids). The tiny probe has a tip size of 50 µm and is integrated in a T-shape flow cell for easy connection via Luer-Lock adapters to external tubings. Liquids (like water, blood, etc.) can be pumped through the cell. MicroImplant The MicroImplant fiber optic oxygen sensor (WPI #501658) is an implantable probe (IMP) with a tiny probe tip size 50 µm, an exposed fiber length of 5-mm and a jacket diameter of 900 µm. The IMP sensor was successfully implanted in crabs, fishes and soil. OxyMini systems The OxyMini is a single-channel fiber optic oxygen meter for WPI’s fiber optic oxygen minisensors. These sensors are based on 2 mm polymer optical fibers and have a length of 2.5 m. A wide range of applications is possible with these sensors. ● Process control: bottling plant in breweries and quality control of packages ● Biotechnology: Control of cell culture media and non-invasive control of bioreactors ● Implantation of oxygen sensors into soil and trees. OxyMini and OxyMicro Benefits ● ● ● ● ● ● ● ● BIOSENSING Oxygen is not consumed during the experiment Immune to electrical and magnetic interference Excellent long-term stability No lengthy polarization necessary (e.g., as Clark-type oxygen electrodes require) Fast response time < 0.5 s for MicroTip sensors Probe size of MicroTip sensors as small as 50 µm Measurement is feasible in dry gas Optical isolation of sensor tip available for fluorescent or photosynthetically active samples MiniTip This oxygen dipping probe (WPI #501641) has a tip diameter of 4 mm and consists of a polymer optical fiber, with an oxygen sensitive coating. The MiniTip’s range is 0 to 100%. This robust sensor has a response time (t90) of approximately 40 s. Measurement Principle Conventional fiber-optic oxygen sensor systems based on intensity measurements are limited in their accuracy by light source stability and ambient light fluctuations. Using a luminescence lifetime detection, measurements are not affected by light source stability, intensity fluctuations caused by fiber bending or changes of the optical properties of the sample (turbidity, refractive index, coloration, etc.). These advantages make WPI’s OxyMini and OxyMicro the most advanced and reliable fiber-optic oxygen system available. Calibration: The sensors can be calibrated by a simple two point calibration, 100% air-saturation and 0% air saturation. OxyMini and OxyMicro oxygen meters: The OxiMini and OxyMicro fiber optic oxygen meters are compact, easy to transport. The instruments are designed for in/outdoor use and can be connected to a PC via a RS232 interface. Data can be visualized, analyzed and stored with the supplied software. A full range of sensors covering most biomedical applications are available. MiniFlow The MiniFlow oxygen probe (WPI #501642) is a miniaturized fiber optic chemical sensor integrated in a T-shape flow through cell. The standard T-shape flow cell can be easily connected via Luer-Lock adapters to external tubings. Liquids (e.g., water, blood, etc.) can be pumped through the cell. The sensor has a response time (t90) of approximately 40-s and an excellent long-term stability. MiniFoil WPI offers the sensor material on a 1 cm2 support disk made of polyester. This material can be glued, for example, inside glass vials and the oxygen concentration can be measured noninvasively and non-destructively from outside through the wall of the flask. A plastic fiber optic cable (WPI #501644, WPI #501645) is used to illuminate the sensor foil. The wall of the flask must be transparent/ non-fluorescent. Response time (t90) of approximately 50 s. The material can be implanted into animal tissues or custom-made housings. OxyMicro systems The OxyMicro is a single channel fiber optic oxygen meter for WPI’s fiber optic oxygen microsensors. Applications include: ● Oxygen profiles of marine sediment, soils, or tissue ● Implantation into living tissue (e.g., heart or muscle tissue) ● Control of cell culture media in Biotechnology. MINISENSOR SYSTEM OXY-MINI-AOT Fiber-optic Oxygen Meter for Minisensors * MINISENSORS (not interchangeable with Microsensors) 501641 MiniTip, fiber-optic oxygen sensor 501642 MiniFlow, flow-through cell with integrated planar oxygen sensor 503090 MiniSpot, planar oxygen-sensitive spot, 5 mm diam. (includes 10) Requires #501644 501644 Polymer optical fiber with 1 SMA connector MICROSENSOR SYSTEM OXY-MICRO-AOT Fiber-optic Oxygen Meter for Microsensors * MICROSENSORS (not interchangeable with Microsensors) 501656 MicroTip, needle-type housing fiber-optic oxygen sensor, 50 µm tip 501656-C MicroTip, needle-type housing, 50 µm tip, optical isolation 501656-F MicroTip, needle-type housing, 140 µm flat tip 501657 MicroFlow, flow-through housed oxygen microsensor 501658 MicroImplant, implantable oxygen microsensor, 50 µm tip 501658-F MicroImplant, 140 µm flat tip *Meter contains two analog outputs and one trigger input MicroTip The MicroTip (WPI #501656) is a needle-type (27 ga.) oxygen micro sensor designed for applications where a small tip size of 50 µm and a fast response time (t90) of 1 s are necessary. The oxygen sensitive sensor tip consists of 140 µm fiber tapered to a 50 µm tip. The sensor is housed inside a stainless steel needle of 22 mm length and 0.4 mm diameter. This allows penetration through a septum rubber or similar material. These sensors are ideal for oxygen profiling in sediments and biofilms. MiniTip Measurement Range dissolved/gaseous Response Time [t90] dissolved/gaseous Sterilization (EtOH, H2O2) autoclavable (130°C, 1.5 atm) Drift (100,000 datapoints, 20°C) Accuracy (20°C) Resolution (20°C) Temperature Range 0-45 ppm, 0-100% 0-760 mmHg 40 s 10 s Y N < 0.1% MiniFlow 0-45 ppm, 0-100% 0-760 mmHg 40 s 10 s Y Y < 0.1% MiniSpot 0-45 ppm, 0-100% 0-760 mmHg 40 s 10 s Y Y < 0.1% 0.2% MicroTip 0-45 ppm, 0-100% 0-760 mmHg <2s < 0.5 s Y N < 0.3% MicroFlow 0-45 ppm, 0-100% 0-760 mmHg <2s < 0.5 s Y Y < 0.3% MicroImplant 0-45 ppm, 0-100% 0-760 mmHg <2s < 0.5 s Y Y < 0.3% 2.75 ±0.01 ppm, 9.00 ±0.05 ppm, 220 ±0.15 ppm, 45.0 ±0.25 mmHg, 150 ±0.75 mmHg, 375 ±2.6 mmHg -10°C to 50°C World Precision Instruments www.wpiinc.com 2.5 m Probe Assembly Length 62 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com