V-Vette Sample holder for spectroscopic analysis of microliter volume samples ● 2 microliter sample ● 1 mm pathlength ● No moving parts ● Baseline repeatability < 2 mAU ● Patent pending V-Vette is a fiber optic sample cell with a pathlength of 1 mm for spectroscopic analysis of microliter volume samples. Light is coupled into and out of the sample cell via optical fibers. A 2 μL sample droplet can be conveniently placed into the v-shaped sample compartment from a pipetter. Absorbance of the sample is measured between the input and output fiber after a cover is placed on the sample compartment to minimize stray light. The sample can be picked up and reused or removed by blowing it off with dry air or wiping it off. V-VETTE V-Vette Microliter Sample Holder FUNCTIONALITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Absorbance COVER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Included PATHLENGTH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 mm WAVELENGTH RANGE . . . . . . . . . . . . . . . . . . . . . . . .200 – 1000 nm FIBER CONNECTION . . . . . . . . . . . . . . . . . . . . . . . . .600 μm (SMA) SAMPLE VOLUME . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5 µL BASELINE REPEATABILITY . . . . . . . . . . . . . . . . . . . .< 2 mAU peak to peak V-VETTE SPECIFICATIONS MicroLWCC SPEC TROS CO PY Low volume flow cell for FIA, HPLC and Process Analysis MicroLWCC is a new fiber optic low volume flow cell for UV/VIS/NIR absorbance analysis. Based on WPI’s established liquid core waveguide technology, the analyte solution functions as the core of a fluid filled light waveguide. Wetted parts in the sample cell light path are PEEK, fused silica and PTFE. Optical fibers are used to transport light to and from the sample cell. The cell can be used in biochemistry for DNA, RNA & protein quantification, colorimetric nutrient and trace metal analysis, drug discovery and dissolution testing, process control, and HPLC analysis. LWCC-M SPECIFICATIONS LWCC-M-10 OPTICAL PATHLENGTH INTERNAL VOLUME REFRACTIVE INDEX @ 280 nm** 10 mm 2.4 µL < 7 mAU LWCC-M-50 50mm 12 µL < 15 mAU LWCC-M-100 100mm 24 µL < 30 mAU LWCC-M-10 LWCC-M-50 LWCC-M-100 Low Volume Flow Cell, 10 mm pathlength Low Volume Flow Cell, 50 mm pathlength Low Volume Flow Cell, 100 mm pathlength References M. Belz, “Simple and sensitive protein detection system using UV LEDs and liquid core waveguides”, Advanced Environmental, Chemical, and Biological Sensing Technologies V, Optics East, Oct 2007, Proc. SPIE, Vol. 6755, 675505 M. Belz, F. A. Klein, H. S. Eckhardt, K. Klein, D. Dinges, K. T. V. Grattan, “Optical Detection Techniques and Light Delivery with UV LEDs and Optical Fibres”, Third International Conference on Optical and Laser Diagnostics, Proc. IOP, City University, London, UK, May 2007. M. Belz, P. Dress, A. Sukhitskiy, S. Liu, “Linearity and effective optical pathlength of liquid waveguide capillary cells”, Part of the SPIE Conference on Internal Standardization and Calibration; Architectures for Chemical Sensors, Boston Massachusetts, September 1999, SPIE Vol. 3856, 271-281. WAVELENGTH RANGE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .200 – 1000 nm FIBER CONNECTION [µm] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500 (SMA) TRANSMISSION @ 254 nm * . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .> 40% MAXIMUM PRESSURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . > 1000 psi WETTED MATERIALS . . . . . . . . . . . . . . . . . . . . . . . . . . . PEEK, Fused Silica, PTFE * Reference: 2 * 600 µm Fiber, butt-coupled ** Measured using ASTM E 685 - 93 WPI U.S. Patents: 5,444,807; 5,570,447; 5,604,587; 6,603,556; 6,385,380. 200 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com