Pneumatic PicoPumps Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps use carefully regulated air pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. A second pressure port maintains a low positive “holding” pressure to the injecting pipette between injection pulses, to prevent fluid uptake through capillary action or diffusion. Timing, ejection pressure, holding Repeatable microinjection in volumes ranging from picoliters to nanoliters pressure, and suction are adjusted independently by control knobs and indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. A built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting. The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse. A 5-volt monitor output provides a logiclevel pulse for your computer or other monitoring device. The most recognized picopump in the world! PUMPS, MICROINJECTION For a complete list of pre-pulled micropipettes, see µTips™, or call us with your special requirements. PV830 — Eject pressure, Hold pressure, and Vacuum are all available, controlled by separate regulators on the front panel. Eject pressure supplies a high-pressure pulse for injecting fluid. Hold pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive. Pressure in the injection pipette is automatically switched between Eject and Hold pressure by a precision timing circuit that controls a solenoid valve. Vacuum is used to fill pipettes from the tip or to secure a floating cell during microinjection. Vacuum is regulated the same way, by a 20-turn knob on the front panel. Vacuum may be switched from regulated vacuum to atmosphere by using the pneumatic toggle on the front panel. Vacuum can also be routed to the eject port. Each PicoPump is supplied with a 5430-ALL kit that includes two PicoNozzles and tubing to connect the holders to the pressure and vacuum ports. SYS-PV820 PicoPump w/ hold pressure SYS-PV830 PicoPump w/ hold pressure and vacuum Specify line voltage All PicoPumps require external vacuum source — see below. OPTIONAL ACCESSORIES 3260 Foot Switch 2932 Rack Mount Kit, 31⁄ 2-in. high (PV820) 2933 Rack Mount Kit, 51⁄4-in. high (PV830) 5430-10 PicoNozzle Kit (MPH6S for 1.0 mm pipette & 5-ft tubing assembly) 5430-12 PicoNozzle Kit (MPH6S for 1.2 mm pipette & 5-ft tubing assembly) 5430-15 PicoNozzle Kit (MPH6S for 1.5 mm pipette & 5-ft tubing assembly) 5430-20 PicoNozzle Kit (MPH6S for 2.0 mm pipette & 5-ft tubing assembly) 5430-ALL PicoNozzle Kit (for 1.0, 1.2, 1.5, and 1.65 mm pipettes & 5-ft tubing assembly) 75122-110 Replacement Gaskets for PicoNozzle — 1.0 mm, green, package of 10 75122-210 Replacement Gaskets for PicoNozzle — 1.2 mm, black, package of 10 75122-310 Replacement Gaskets for PicoNozzle — 1.5 mm, blue, package of 10 75122-410 Replacement Gaskets for PicoNozzle — 1.65 mm, red, package of 10 MPH6S Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm) MPH6R Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm) 3316 Replacement Input Kit New PicoNozzle Kit 5430-ALL (included) allows micropipettes to be securely mounted in micropositioners for stable axial air delivery. Because air enters the pipette axially, lateral whipping during injection is eliminated.   172 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com